T细胞亚群水平检测对判断新生儿巨细胞病毒感染活动性意义.docVIP

T细胞亚群水平检测对判断新生儿巨细胞病毒感染活动性意义 　　[摘要] 目的 探讨巨细胞病毒感染对新生儿细胞免疫的影响，为新生儿巨细胞病毒感染的防治提供依据。 方法 选择2008年5月～2010年10月本院巨细胞病毒感染新生儿38例，其中18例出现感染性症状（A组），20例未出现感染性症状（B组），选取同期健康新生儿20例（C组），分别采集三组患儿静脉血，检测患儿T细胞亚群水平。 结果 A组患儿CD3+，CD4+，CD4+/CD8+明显低于B组及C组（P0.05）；A组CD8+水平明显高于B组、C组，差异有统计学意义（P0.05）。 结论 巨细胞病毒感染患儿出现症状性感染者存在细胞免疫功能异常，临床检测新生儿T细胞亚群水平对判断新生儿巨细胞病毒活动性感染、指导早期治疗有重要意义。 　　[关键词] 巨细胞病毒；新生儿；细胞免疫；活动性感染 　　[中图分类号] R272.1 [文献标识码] B [文章编号] 1673-9701（2012）01-084-02 　　 　　Significance of T-cell subsets detection on the judging of neonatal active cytomegalovirus infection 　　QIAO Zengpei LIU Caixia XIE Qipeng 　　Department of Laboratory, The Second Affiliated Hospital of Wenzhou Medical College, Wenzhou 325027, China 　　 　　[Abstract] Objective To investigate the effect of cytomegalovirus (CMV) infection on neonatal cellular immunity, and to provide the basis for the prevention and treatment of CMV infection. Methods All of 38 cases of neonatal cytomegalovirus infection in the hospital were selected, from May 2008 to October 2010. Of which 18 patients had symptoms of infection (Group A), 20 patients with no infectious symptoms (Group B), and 20 cases of healthy newborns were selected in the same period (Group C). Venous blood samples of the three groups were collected to detect T-cell subsets. Results CD3+, CD4+ and CD4+/CD8+ of patients in group A were significantly lower than that group B and group C (P0.05). CD8+ level of group A was significantly higher than that the group B and group C (P0.05). Conclusion The neonatal with symptomatic cytomegalovirus infection showed cellular immune dysfunction, so it is very important to detect T cell subsets of neonatal to discern active cytomegalovirus and guide primary treatment. 　　[Key words] Cytomegalovirus; Neonatal; Cellular immunity；Active infection 　　 　　巨细胞病毒（CMV）是一种疱疹病毒，分布广泛，CMV感染可引起泌尿生殖系统、中枢神经系统、肝脏、肺、血液循环系统等病变，并与恶性肿瘤的发生可能有关。感染各种病菌而引起的疾病是新生儿发病、死亡的主要原因，其中巨细胞病毒感染是胎儿在子宫内发生感染的主要原因之一，也是引起新生儿先天畸形的主要原因[1]。新生儿巨细胞病毒感染主要分为先天感染及后天感染；调查显示，目前我国新生儿CMV先天感染占4%左右。C