费菜和地锦草生物活性成分分析-analysis of bioactive components in cauliflower and euphorbia humifusa.docxVIP

摘 要本论文分为 3 章。第一章采用活性追踪的方法对费菜和地锦草 2 种植物进行化学成分的研究，共分离得到 25 个化合物，鉴定了其中的 13 个化合物；第二章对 2 种植物的 不同溶剂提取物进行抑制α-葡萄糖苷酶活性研究、体外抗氧化作用研究和抗菌作用研 究；第三章对大戟属植物的化学成分、药理作用及生态价值进行了综述，以期对其进行 更好的保护和合理利用。一、费菜和地锦草化学成分研究对来源于景天科景天属植物费菜(Sedum aizoon)全草化学成分进行研究，从乙酸乙酯 部位分离得到 3 个化合物，鉴定了其中的 2 个化合物；从正丁醇部位分离得到 8 个化合物，鉴定了其中的 4 个化合物。对来源于大戟科大戟属植物地锦草(Euphorbia humifusa)全草化学成分进行研究，从 乙酸乙酯部位分离得到 4 个化合物，鉴定了 4 个化合物；从正丁醇部位分离得到 8 个化合物，鉴定了其中的 3 个化合物。 二、生物活性研究1.利用建立的 α-葡萄糖苷酶抑制剂体外微量筛选模型，首次对费菜和地锦草 2 种植 物的不同提取溶剂提取物 α-葡萄糖苷酶抑制活性进行研究。费菜石油醚提取物、乙酸乙 酯提取物、正丁醇提取物、总甲醇提取物及地锦草石油醚提取物、乙酸乙酯提取物、正 丁醇提取物均有很好 α-葡萄糖苷酶抑制活性。2. 首次采用 DPPH、ABTS 和 FRAP 3 种方法对费菜和地锦草的不同溶剂提取物进 行抗氧化活性的综合评价。发现费菜乙酸乙酯提取物、正丁醇提取物和总甲醇提取物及 地锦草乙酸乙酯提取物和正丁醇提取物都具有很好的抗氧化活性。3. 首次对费菜和地锦草 2 种植物的提取物的抗菌活性进行研究。通过对费菜和地 锦草各提取物对 SA、MRSA、ESBLs-SA 的抗菌活性研究，发现地锦草较费菜抗菌效果 好，抗菌活性主要存在于石油醚部位。三、大戟属植物研究进展 本章分别从化学成分、药理作用及生态价值等方面对大戟属植物进行概述，以期对其进行更好的保护和合理利用。关键词：费菜；地锦草；化学成分；生物活性ABSTRACTThis dissertation is composed of three chapters. The first chapter showed the isolation and identification of all kinds of compounds from Sedum aizoon and Euphorbia humifusa by bioassay guide. Twenty-three compounds were isolated and thirteen compounds were identified. The second chapter reported α-glucosidase inhibitory activity , antioxidant and antibacterial action in vitro of extracts from S. aizoon and E. humifusa. In the last chapter, the study progress of chemical constituent, pharmaceutical and ecological value of the Euphorbia L. were summarized.Study on the chemical constituents of S. aizoon and E. humifusaSedum aizoon belongs to Crassulaceae and Sedum family, and three compounds were isolated from ethyl acetate extract and two compounds were identified. Eight compounds were isolated from n-butanol extracts and four of which were identified.Euphorbia humifusa belongs to Euphorbiaceae and Euphorbia L. family, and four compounds were isolated from ethyl acetate extract and four compounds were identified. Eight compounds were isolated from n-butanol extracts and three of which were identified.Study on biolo