凤尾草总黄酮对大鼠前列腺增生的干预作用及机制分析-intervention effect and mechanism analysis of total flavonoids from pteris multifida on prostatic hyperplasia in rats.docxVIP

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凤尾草总黄酮对大鼠前列腺增生的干预作用及机制分析-intervention effect and mechanism analysis of total flavonoids from pteris multifida on prostatic hyperplasia in rats.docx

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凤尾草总黄酮对大鼠前列腺增生的干预作用及机制分析-intervention effect and mechanism analysis of total flavonoids from pteris multifida on prostatic hyperplasia in rats

Intervention effect and mechanism research of total flavonoidof Pteris multifida in benign prostatic hyperplasia rats AbstractObjective：Research the intervention effect of total flavonoid of Pteris multifida inbenign prostatic hyperplasia(BPH) castrated rats and the possible mechanism,to provide experimental basis for further experimental study and clinical application in the future.Methods: Removing of bilateral testes in SD rats, and subcutaneous injecting of testosteronepropionate,establishedcastratedratmodelofbenignprostatichyperplasia.After the success of modeling，The model rats Were randomly divided into 6groups，The Total Flavonoid of Pteris Multifida group (high, medium, low dose group), positive drug group (dutasteride group, prostat group), and simple model group, separate a blank control group，respectively were fed with bracken (30mg/kg), middle dose (60 mg/kg), high dose (120mg/kg) of total flavonoid of Pteris multifida,positive drug dutasteride (0.1mg/kg) and prostat tablets (20mg/kg),blank control group and model group were fed with the same volume of distilled water.Every group was given daily administration and consecutive for 45 days.24 hours after the last administration,rats were killed, isolated the prostate, weighed and calculated of prostatic index (PI), measured the level of Serum testosterone (T),dihyrotestotestosterone (DHT), estradiol (E2). Observe the pathological changes at the prostate tissue under the light microscope,observe the ultrastructural changes of prostate cells under the Electron microscope.Result:Subcutaneous injection of testosterone propionate can reproduce the model of BPH in rats successfully,the prostate wet weight, prostatic index were significantlyincreased (P 0.01), the glandular epithelium of BPH model changed to high columnar,cells packed tightly, part of the glandular epithelium hyperplasia like papillary and broke into the glandular cavity and showed change like pseudostratified, individual cells can be