中医辨证治疗对NAFLD大鼠血脂等影响实验研究.docVIP

中医辨证治疗对NAFLD大鼠血脂等影响实验研究 　　摘 要：目的：探讨中医辨证治疗对非酒精性脂肪性肝病(NAFLD)大鼠各时期血脂等指标影响的作用机制，为临床防治NAFLD提供理论依据。方法：取SPF级Wistar大鼠72只，雌雄各半，随机分为正常组、模型组、对照药物治疗组、药物A方治疗组、药物B方治疗组、先A方后B方治疗组、先B方后A方治疗组、先空白后A方治疗组、先空白后B方治疗组，共9组。除正常组给予普通饲料外，其余各组均饲以高脂饲料。造模第4周后，模型组加用生理盐水灌胃；对照组加用对照药物灌胃；药物A方组加用治疗药物A方灌胃；药物B方组加用治疗药物B方灌胃；先A方后B方治疗组即4~8周用A方，8~12周用B方灌胃；先B方后A方治疗组即4~8周用B方，8~12周用A方灌胃；先空白后A方治疗组即先空白，第8~12周用A方灌胃；先空白后B方治疗组即先空白，第8~12周用B方灌胃。实验第12周时，测定大鼠血清TCH、TG、HDL、LDL、FFA、GSH-ST、TNF-α的值。结果：实验第12周时，先A方后B方治疗组(编号为6组)降低NAFLD后期大鼠血清TG、TCH、LDL、FFA、TNF-α及升高血清GSH-ST水平的疗效与其他治疗组相比尤为显著(P 　　? 　　关键词：中医多法治疗；血脂；NAFLD；TNF-α；GSH-ST? 　　中图分类号：R-33 　　文献标识码：A文章编号： 　　1673-7717（2008）11-2402-04 　　?? 　　Experimental Study on Effect of Traditional Chinese Medicine Dialectical Treating?Blood-fat Index on NAFLD Rats 　　?? 　　LI Yun?cheng?1, SHENG Guo?guang?2, HUANG Yu?hua?2, XU Jian?liang?2? 　　（1. Hubei College of Traditional Chinese Medicine,Wuhan 430061，Hubei，China; ?2. Institute of Liver Disease, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan 430061,Hubei，China 　　）?? 　　 　　Abstract：Objective：To explore the function mechanism of traditional Chinese medicine dialectical treating blood-fat index and so on in nonalcoholic fatty liver disease (NAFLD) rats various times, which provide the basis theory to prevent and cure the NAFLD in clinic.Methods：Fetched 72 wistar rats level of SPF, females and males were half and half, were divided into 9 groups at random: Normal group, Model group, Comparison medicine treatment group, Medicine A treatment group, Medicine B treatment group, First Medicine A then Medicine B treatment group, First Medicine B then Medicine A treatment group, First blank then Medicine A treatment group, First blank then Medicine B treatment group. Except the normal group was fed ordinary diet, the other eight groups were fed by high fat and high Cholesterol diet. After 4 weeks, Model group rats were given physiological saline by intragastric administration. Comparison medicine treatment group rats were