中等强度运动后大鼠不同部位组织荧光光谱研究.docVIP

中等强度运动后大鼠不同部位组织荧光光谱研究 　　摘 要:为了研究运动训练大鼠不同部位组织(重点是脂肪组织)和器官的 自体荧光光谱特 性。方法:通过激光诱导荧光光谱技术测量一次中等强度力竭运动后大鼠组织的自体荧光光 谱,并在肠系膜部位的脂肪组织的三维荧光光谱中发现了和运动相关的特有荧光峰,并判别 出此荧光峰对应的荧光物质是NADH(还原态烟酰胺腺嘌呤二核苷酸),且研究了运动状态与 峰强的相关性。结果:中等强度运动后大鼠肠系膜部位的脂肪组织的能量代谢强于大网膜部 位的脂肪组织、皮下脂肪组织,并且强于前腿肌肉、比目鱼肌及其它脏器; NADH自体荧光 光谱特性是判断机体组织代谢程度的有效的指标之一。 　　关键词:自体荧光;荧光光谱;NADH;不同部位;脂肪组织 　　中图分类号:G804.7文献标识码:A文章编号 :1007-3612(2009)08-0051-03 　　 　　A Study the Autofluorescence Spectrum of Tissues in Different Pl aces of Rats after Mid?intensity Exercise 　　REN Wen?jun?1, ZHANG Bin?nan?2, XU Zheng?hong?3, ZHANG Zhen ?xi?1, LI Zheng?1 　　(1. Science and Technology, Xian Jiaotong University, Xian 　　71 0049, Shaanxi China; 　　2. PE Department, Xian Jiaotong University, Xian 　　710049, Shaanxi China; 　　3. School of Electrical Engineering, Xian Jiaot ong Unive rsity, Xian 　　710049, Shaanxi China) 　　Abstract: Aim: to investigate three?dimensional autofluorescence spectral charac teristics of tissues and organs of different places of exercising rats. Methods:By laser?induced fluorescence (LIF) technique, three?dimensional autofluoresc en ce spectral characteristics of tissues and organs of different places of exercis ing rats after treadmill exercise with 　　5° incline, 　　32m/min,180 min/time are st udied. Results: Specific exercise?related fluorescence peaks are discovered int he three?dimensional fluorescence spectra of adipose tissue and fluorescent sub s tance corresponding to the fluorescence peaks is determined to be nicotine?amid eadenine dinucleotide reduced(NADH). The study indicates that the energy metabol ism of rats adipose tissue is stronger than other organs and the autofluorescen ce spectral characteristic is one of the effective indexes to judge the metaboli c of muscle. 　　Key words: autofluorescence; fluorescence spectrum; NADH; different plac es; adipose tissue 　　 　　近年来,运动干预肥胖成为运动康复研究的热点,而有氧练习对身体不同部位的脂肪组织的 代谢影响的研究少见报道,应用激光诱导荧光光谱技术研究机体脂肪组织代谢的报道更少见 。激光诱导荧光光谱包括内源荧光物的自体荧光光谱及外源荧光物或荧光前驱物的荧光光谱 [1-3]两大类。两者相比而言,自体