Eag钾离子通道在宫颈癌中的表达以及缺氧对其的调控的论文.docVIP

Eag1钾离子通?道在宫颈癌中的表?达以及缺氧对其的?调控的论文 Ea?g1钾离子通道在?宫颈癌中的表达以?及缺氧对其的调控?的论文 eag1?钾离子通道在宫颈?癌中的表达以及缺?氧对其的调控 ? ?【关键词】 ? ? 宫颈癌;钾离?子通道;eag1?（本站文章仅给需?要的医务工作者提?供交流学习参考。?翰林医学论文网免?费提供。部分资源?由工作人员网上搜?索整理而成，如果?您发现有哪部分资?料侵害了您的版权?，请速与我们后台?工作人员联系，我?们将即时删除。客?服qq：8897?0242.后台工?作qq：9283?33977） ?【摘要】 ? 目的 研究不?同病变宫颈组织中?eag1表达情况?以及缺氧对宫颈癌?hela细胞膜上?eag1钾离子通?道表达和活性的影?响。方法 采用免?疫组化法检测不同?宫颈组织和宫颈癌?hela细胞中e?ag1蛋白的表达?情况;采用rt-?pcr法检测不同?缺氧时间和缺氧浓?度对hela细胞?中eag1 mr?na表达的影响。?采用全细胞膜片钳?记录的方法，研究?缺氧对eag1钾?离子通道活性的影?响。结果 宫颈上?皮内瘤变组织中e?ag1蛋白的表达?明显高于正常组，?宫颈癌组织中ea?g1的表达明显高?于宫颈上皮内瘤变?组;eag1的表?达随临床分期的增?加逐渐增高，ea?g1的表达与病理?分级无明显相关性?。正常培养的宫颈?癌hela细胞中?即有eag1表达?;其表达随缺氧程?度加深及缺氧时间?的延长明显增强。?hela细胞中e?ag1钾离子通道?是电压依赖性的，?具有延迟整流及不?失活特性;轻度缺?氧条件能使hel?a eag1钾离?子通道电流强度增?大、激活时间缩短?。Www..Co?M结论 eag1?钾离子通道蛋白有?可能成为新的早期?筛查和判断宫颈癌?生物学行为的诊断?指标。 ? 【关?键词】 ? ?宫颈癌;钾离子通?道;eag1 e?xpressio?n of eag?1 k+ cha?nnel in ?cancer o?f the ce?rvix and? the reg?ulation ?of hypox?ia lin s?huang,li? li,liu ?yuan,et ?al. depa?rtment o?f obstre?ics and ?gynecolo?gy,dapin?g hospit?al,the t?hird mil?itary me?dical un?iversity?,chongqi?ng 40004?2,china ?[abstrac?t] objec?tive imm?unohisto?chemistr?y was em?ployed t?o detect? the exp?ression ?of eag1 ?protein ?in diffe?rent cer?vix uter?i tissue?s and ca?cx hela ?cells.me?thods we? determi?ned the ?effect o?f differ?ent hypo?xia time? and hyp?oxic con?centrati?on on th?e expres?sion of ?eag1 mrn?a in hel?a cell u?sing rt-?pcr. mea?nwhile, ?whole ce?llular p?atch cla?mp recor?ding was? employe?d to inv?estigate? the eff?ect of h?ypoxia o?n activi?ty of ea?g1 k+ ch?annel.re?sults th?e expres?sion of ?eag1 pro?tein in ?cervical? intraep?ithelial? neoplas?ia (cin)? tissue ?was sign?ificantl?y higher? than th?at in no?rmal con?trol, an?d the ex?pression? in cacx? tissue ?was rema?rkably h?igher th?an that ?in cin g?roup. th?e expres?sion of ?eag1 asc?ended as? the dev?elopment? of clin?ical sta?ge of ce?rvical c?ancer, b?ut almos?t irrele?vant to ?patholog?ical gra?de. resu?lts of i?mmunoflu?or