野木瓜片中绿原酸含量测定.docVIP

野木瓜片中绿原酸含量测定 　　[摘要] 目的:建立野木瓜片质量标准中的含量测定方法。方法:用HPLC法测定野木瓜片中绿原酸的含量,以十八烷基键合硅胶为固定相,乙腈∶0.1%磷酸(体积比11∶89)为流动相,流速为1 ml/min,测定波长为327 nm。结果:绿原酸在10.21～102.1 μg/ml浓度范围内有良好的线性关系(r=1.000 0),回收率为98.90%(RSD=0.8%)。结论:本法测定野木瓜片中的绿原酸含量,结果稳定、准确可靠,可作为该品种的质量控制方法。 　　[关键词] 野木瓜片;绿原酸;高效液相色谱法 　　[中图分类号] R927.2 [文献标识码]C [文章编号]1674-4721(2010)04(a)-114-02 　　 　　Determination of chlorogenic acid in Caulis stauntoniae cbinensis tablet 　　LI Guogiang1,2, ZHOU Benhong1, WU Guoqiu2, ZHANG Zhijian2 　　(1.Wuhan University,Wuhan 430072,China;2.Guangzhou Huacheng Pharmaceutical Factory, Guangzhou 510555,China) 　　[Abstract] Objective:HPLC was used to determine the content of chlorogenic acid in Caulis stauntoniae cbinensis tablet. Methods:The study indicated that the effects of separation and determination were optimum when deter-mination parameters were as follows:analytic column:Shim-pack VP-ODS column,the mobile phase was acetonitrile-0.1% Phosphate (11∶89), The flow rate was 1 ml/min; UV detectionwavelength was 327 nm. Results:The repressive equation had a good linear relationship (r=1.000 0). The relationship of injection amounts and peak was linear in the range of 10.21-102.1 μg/ml and recovery rate was 98.9%(RSD=0.8%).The method was simple and available,accuracy and good reproducibility. Conclusion:This Caulis stauntoniae cbinensis tablet determination of chlorogenic acid content,the results are stable, accurate and reliable,can be used as the variety of quality control methods. 　　[Key words] Caulis stauntoniae cbinensis tablet;Chlorogenic acid; HPLC 　　 　　野木瓜片为木通科植物野木瓜的干燥茎及叶经水提、浓缩、干燥成干膏后制成颗粒压制成片而得。其功能与主治为祛风止痛、舒筋活络。用于风邪阻络型三叉神经痛,坐骨神经痛,风湿关节痛。野木瓜含有多种化学成分,主要有去甲五环三萜皂苷类化合物、木脂素苷类化合物、黄酮类和酚性成分等。野木瓜片原标准收载于部颁中药14册,标准号为WS3-B-2777-97,原标准只对黄酮类进行显色鉴别及以野木瓜对照药材进行薄层鉴别。本文取用高效液相色谱法测定野木瓜片中绿原酸的含量,并进行方法学研究。 　　1 资料与方法 　　1.1 仪器与试药 　　SHIMADZU SPD-10A 型高效液相色谱仪,HW色谱工作站,绿原酸对照品由中国药品生物制品检定所提供(110753-200413),乙腈为色谱纯,其他试剂均为分析纯,野木瓜片(广州市花城制药厂生产)。 　　1.2 色谱条件 　　色谱柱:Hypersil BDS C18柱(4.6 mm×250 mm,5 μm),流动相:乙腈∶0.1%磷酸(11∶89),流