失血性休克大鼠体外实验关于内皮细胞蛋白C系统研究.docVIP

失血性休克大鼠体外实验关于内皮细胞蛋白C系统研究 【摘要】目的 观察失血性休克大鼠血清调节内皮细胞表达凝血-抗凝分子TM和EPCR的作用。方法 本实验于温州医学院实验室完成，选取成年健康SD大鼠30只随机（随机数字法）分成3组：健康对照组、实验对照组（假休克组） 和实验休克组（失血性休克组），每组均为10 只。采用颈内动脉均放血至40 mm Hg（1 mm Hg=0.133 kPa） 60 min建立失血性休克动物模型，留取假休克组、休克组血清，并将胎牛血清作为健康对照组，无菌处理后加入到培养基，与内皮细胞分别培养6 h、12 h和18 h，用逆转录-聚合酶链反应检测休克血清刺激后内皮细胞TM、EPCR mRNA的表达。结果 与健康对照组相比，休克组血清刺激SVAREC 6 h后，TM和EPCRmRNA均升高（P【关键词】失血性休克；血栓调节素；内皮细胞蛋白C受体；凝血障碍；聚合酶链反应An in vitro study of thrombomodulin-protein C-EPCR system coagulation function in hemorrahgic shock rats SHEN Ye，CAI Wen-wei，WU Shuang-hua，ZHANG Mei-qi，PAN Jing-ye.Department of Emergency， Zhejiang Provincial People’s Hospital， Hangzhou 310014，ChinaCorresponding author：PAN Jing-ye，Email：panjingye@hosp1.ac.cn【Abstract】Objective To investigate the coagulant function of Thrombomodulin-Protein C-EPCR System in rats with hemorrhagic shock in virto study.Methods In this study， 30 rats were divided into three groups，with each group 10 rats： control group， the sham shock group， hemorrhagic shock group.Hemorrhagic shock model was subjected to computer-controlled arterial hemorrhage to 40 mm Hg for 60 min .The rat blood serum samples were obtained from rats in sham shock group and shock group after maintaining 3 hours， and fetal bovine serum（FBS） was used as control group. Then medium contained fore-mentioned serums cultured with SV40-transformed aortic rat endothelial cellsfor 6 hours， 12 hours and 18hours. The messenger RNA（mRNA） expression of thrombmodulin（TM）， Endothelial cell protein C receptor in the SV40-transformed aortic rat endothelial cells which were treated with serums were detected by reverse-transcription polymerase chain reaction.Results Compared to control group， SVARECs were incubated for 6hrs with shock serum， the mRNA expression of TF， TM and t-PA was significantly higher， and continued elevating at the time of 18 hours（P【Key words】Hemorrhagic shock： Thrombomodulin； Endothelial cell protein C receptor； Coagulation disorders ； Polymerase chain reaction.蛋白C途径是机体抗凝系