葛根素预处理对兔急性心肌缺血-再灌注损伤时心梗范围及心肌IL-6、TNF-α的影响.docVIP

　　葛根素预处理对兔急性心肌缺血/再灌注损伤时心梗范围及心肌IL-6、TNF-α的影响 作者：艾凌云,吴延庆,宋小敏,陈琦,肖鲁闽,张诗渊,程晓曙,吴清华,苏海 【摘要】 目的 观察葛根素预处理对兔急性心肌缺血再灌注心肌的保护作用。方法 新西兰大白兔60只,分为5组。S组：假手术组；C组：实验对照组；P组：葛根素组；F组：氟伐他汀组；P+F组：联合给药组(葛根素+氟伐他汀),每组12只。建立心肌缺血/再灌注模型,分析心肌梗死范围,用ELISA法测定心肌肿瘤坏死因子(TNF-α)、白细胞介素6(IL-6)。结果 与C组相比,P、F、P+F组心梗体积比明显减少(Plt;0.01)；F组减少心梗体积比作用大于P组(Plt;0.05)；P+F组比P、F组减少心肌梗死体积作用显著(Plt;0.05)。非梗死区心肌IL-6、TNF-α含量比较：C、P、F、P+F组明显高于S组(Plt;0.01)；P、F、P+F组明显低于C组(Plt;0.01)；F组低于P组(Plt;0.05)；P、F组与P+F组高于P+F组(Plt;0.05)。结论 兔急性心梗再灌注时非梗死区心肌IL-6、TNF-α浓度升高；葛根素短期预处理可以抑制炎症反应,减少心梗体积比；氟伐他汀作用大于葛根素；葛根素联合氟伐他汀作用大于单用葛根素或氟伐他汀。 【关键词】 葛根素；氟伐他汀；心肌缺血；再灌注损伤 　　Abstract：Objective To investigate the protective effect of Puerarin and/or Fluvastatin pretreatment on myocardium suffered from rabbit acute myocardial ischemia/reperfusion injury (IRI). Methods Sixty rabbits were divided to 5 groups randomly (n=12)：the sham-operated group (S group), the control group (C group), Puerarin group (P group), Fluvastatin group (F gruop), Puerarin + Fluvastatin group (P+F group). Myocardial is Chemia/reperfusion model was made and AMI aeas were analyzed by photoshop 8.0. TNF-α, IL-6 of cardic muscle and blood were evaluated by ELISA method. Results The volume ratio of infarction was obviously decreased in experiment group compared with C group (Plt;0.01). F group decreased the volume ratios of infarction was better than the P group (Plt;0.05). P, F group increased the volume ratio of infarction better than P+F group (Plt;0.05). The level of the IL-6, TNF-α were decreased in experiment group and C group compared with S group (Plt;0.01). The experiment group obviously decreased the level of IL-6, TNF-α compared with C group (Plt;0.01). F group decreased the level of IL-6, TNF-α better than the P group (Plt;0.05). P group or F group increased the level of IL-6, TNF-α better than P+F group (Plt;0.05). Conclusion IRI contribute to the increase of TNF-α and IL-6 in rabbit myocadium. Either short-term pretreatment of Puerarin or Fluvastatin could decrease the volume