丙泊酚对Wistar大鼠肝癌模型中Bax与Bcl—2表达影响.docVIP

丙泊酚对Wistar大鼠肝癌模型中Bax与Bcl—2表达影响 　　【摘要】 目的：探讨不同浓度丙泊酚对Wistar大鼠肝癌模型中凋亡促进基因Bax和凋亡抑制基因Bcl-2表达的影响。方法：将50只雄性Wistar大鼠按随机数字表法分为五组，每组10只，分别为正常对照组（A组），肝癌模型组（B组），低剂量丙泊酚（3 mg/kg）组（C1组），中等剂量丙泊酚（6 mg/kg）组（C2组），高剂量丙泊酚（12 mg/kg）组（C3组）。给药结束后24 h处死大鼠，取肝脏标本观察肝脏特征，并做组织切片行苏木精-伊红（HE）染色及S-P免疫组化检测Bcl-2及Bax的表达，TUNEL法检测肝细胞凋亡指数（AI）。结果：（1）大体标本检查及HE染色：与A组比较，B组大鼠肝脏表面粗糙，部分肝叶表面出现黄色斑点，肝细胞脂肪变性，点状坏死及炎细胞浸润，少量胶原纤维增生；与B组比较，C1、C2、C3组肝细胞变性坏死以及胶原纤维增生程度有所减轻；（2）TUNEL检测结果：随丙泊酚剂量增加，肝癌细胞中凋亡细胞逐渐增多，AI逐渐递增，具有显著差异性（P 　　【关键词】 丙泊酚； 肝癌； 细胞凋亡； Bcl-2； Bax 　　The Influence of Propofol on Expressions of Bcl-2 and Bax in Wistar Rat Model with Hepatic Carcinoma/LIAN Zi-min，CAO Ding-rui，WANG Hong-yuan，et al.//Medical Innovation of China，2014，11（12）：028-030 　　【Abstract】 Objective： To investigate the influence of propofol with different concentrations on the expression of Bcl-2 and Bax in Wistar rat model of hepatic carcinoma. Method：50 Wistar rats were divided into 5 groups according to random number table method， each group had 10 rats， the normal group （the group A）， the liver cancer model group （the group B）， the low dose Propofol group： 3 mg/kg（the group C1）， the moderate dose Propofol group： 6 mg/kg （the group C2）， the high dose Propofol group： 12 mg/kg （the group C3）. The liver tissue were collected in 24 h after the last injection and observed by morphology and hematoxylin and eosin（HE）staining. S-P immunohistochemistry was used to examine the expression of Bcl-2 and Bax in liver tissue of different groups of Wistar rats. Result： （1） Morphology and HE staining： compared with the group A， the group became B ragged and yellow spots appeared on some surfaces. Liver cells appeared steatosis， spotty necrosis and inflammatory cell infiltration by HE staining. Compared with the group B， there were less steatosis and spotty necrosis in the group C （C1， C2， C3）. （2） TUNEL staining showed that increased the dosage of Propofol， apoptosis cells of liver cancer cells increased， the AI was increased gradually， the difference was st