中国冬小麦品种多酚氧化酶活性基因等位变异检测及其分布规4.docVIP

中国冬小麦品种多酚氧化酶活性基因等位变异检测 及其分布规律研究 肖永贵1夏先春1何中虎1, （1中国农业科学院作物科学研究所国家小麦改良中心，北京 100081； CIMMYT中国办事处，北京 100081）Molecular Identification and Distribution of the Polyphenol Oxidase Genes in Chinese Winter Wheat Cultivars XIAO Yong-gui1, HE Xin-yao1, LIU Jian-jun3, SUN Dao-jie4, XIA Xian-chun1, HE Zhong-hu1,2 (1Institute of Crop Sciences, National Wheat Improvement Center/The Nation Key Facility for Crop Gene Resource and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081; 2CIMMYT China Office, Beijing 100081; 3Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100; 4Northwest Agriculture and Forestry University, Yangling 712100, Shannxi) Abstract:【Objective】The aims of this study were to characterize the allelic distribution of PPO genes in Chinese winter wheat cultivars with molecular markers and analyze the association between PPO activity and different PPO alleles for the improvement of PPO activity in marker-assisted selection (MAS).【Method】A total of 311 Chinese wheat cultivars and advanced lines collected from four winter wheat regions (Experiment 1) and common parents in Shandong province (Experiment 2) were tested with the functional markers PPO18, PPO16 and PPO29 for the identification of four allelic variants Ppo-A1a, Ppo-A1b, Ppo-D1a and Ppo-D1b on chromosomes 2A and 2D.【Result】PPO18 could amplify a 685-bp and an 876-bp fragments in the cultivars with the allele Ppo-A1a (high PPO) and Ppo-A1b (low PPO), respectively. Two complementary dominant markers, PPO16 and PPO29, amplified a 713-bp and a 490-bp fragment in the genotypes with Ppo-D1a (low PPO) and Ppo-D1b (high PPO), respectively. The frequencies for Ppo-A1a, Ppo-A1b, Ppo-D1a and Ppo-D1b were 41.8%, 58.2%, 59.8% and 40.2%, in the 311 cultivars and advanced lines, respectively. Significant differences in PPO activities were detected between the genotypes with Ppo-A1a and those with Ppo-A1b (P＜0.05), and between the genotypes for Ppo-D1a and those for