透明质酸钠对膀胱黏膜损伤后修复影响-effect of sodium hyaluronate on repair of bladder mucosa injury.docxVIP

安徽医科大学硕士学位论文 安徽医科大学硕士学位论文 5 5 perfused with large and small (1 650 ku, 1 100 ku) molecular weight, high and low (0.2%, 0.08%) concentrations of SH solution. With conventional staining techniques, we observed the pathological bladder mucosal injury and inflammatory infiltration, and with the technique of immunohistochemistry, SH of heat shock protein 60, 70 (HSP60, HSP70) expressing in the mucosa affected. Results： Endoscopic pathological changes,C, D, E group had mucosal hyperemia, a small amount of bleeding, F group, scattered in small punctate hemorrhage.Concerning the results of HSP60 expression, F group, when compared with B, C groups, There was a significant with them (P 0.01), and when compared with D, C groups it was a significant (P 0.05), and when compared with F, E groups it showed no significant （P0.05）. Concerning HSP70 expression, F group, compared with the B, C, D, E group, showed a significant (P 0.01), and compared with D, C groups it showed a significant (P 0.05). Conclusion： Increasing molecular weight and concentration of SH evidently improved the repair capability of bladder mucosa after injury, which might be related to an augment of the viscosity of the SH. Key words： cystitis?drug therapy; sodium hyaluronate?therapeutic use; molecular weight; concentration ; HSP60; HSP70. 1 1 英文缩略词表 英文缩写 英文名称 中文名称 SH Sodium hyaluronate 透明质酸钠 HSP Heat shock protein 热休克蛋白 HA Hyaluronic acid 透明质酸 CS Chondroitin sulfate 硫酸软骨素 GAG Glycosaminoglycan 葡萄糖氨基聚糖 ECM Extracellular matrixc 细胞外基质 UTI Urinary tract infection 尿路感染 IC Interstitial cystitis 间质性膀胱炎 PVAS Pain visual analog scale score 疼痛直观类比标度评分 S-P Streptavidin-Peroxidase 链霉菌抗生物素蛋白-过氧化酶 DAB Diaminobenzidine 二氨基联苯胺 PBS Phosphate buffered saline 磷酸盐缓冲液 H2O2 Hydrogen peroxide 过氧化氢 SPSS Statistical program for social sciences 社会科学统计程序 6 6 透明质酸钠对膀胱黏膜损伤后修复的影响 1 前 言 自从上世纪70年代就有学者提出膀胱屏障理论，认为膀胱黏膜上皮细胞间的 紧密连接，特殊的伞状细胞，有助于阻挡细菌、某些分子和离子的作用。这层膀 胱屏障主要由膀胱黏膜固有层表面分布的葡萄糖氨基聚糖来实现的[4-5]，葡萄糖 氨基聚糖与蛋白聚糖共同组成的多糖-蛋白聚合物细胞被膜覆盖于膀胱表面[5-7]，