线粒体cx43在动脉粥样硬化中作用与机制-role and mechanism of mitochondrial cx43 in atherosclerosis.docxVIP

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线粒体cx43在动脉粥样硬化中作用与机制-role and mechanism of mitochondrial cx43 in atherosclerosis.docx

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线粒体cx43在动脉粥样硬化中作用与机制-role and mechanism of mitochondrial cx43 in atherosclerosis

Abst Abstract IV IV ABSTRACT Objectives: To explore the effect of mitochondrial Cx43 on atherosclerosis(AS) and its signaling pathway mechanisms. Methods: Two experimental models were established. ① Rat VSMCs proliferation model was induced by Using PDGF-BB, VSMCs were randomly divided into 3 groups: a. control; b.PDGF-BB; c.PD98059+PDGF-BB. ② VSMCs-ECs Co-culture model was established to simulate AS in vitro. Cells were randomly divided into 2 groups: a.control, b.TNF-α. VSMCs activity was detected by MTT assay; Reactive oxygen species(ROS) was checked by Fluorescent probes; Expression of mitochondrial Cx43 was studied by western blot. Results: Rat VSMCs proliferation model: ① MTT assay results showed that: The absorbance value (A value) of PDGF-BB group was increased compared with that of control group(P0.05); A value of PD98059+PDGF-BB group was decreased compared with control group(P0.05); A value of PD98059+PDGF-BB group was decreased compared with PDGF-BB group(P0.01). ② ROS was detected by Fluorescent probes.The results showed that the intensity of fluorescence was proportional to the amount of ROS. The green fluorescence of PDGF-BB group was stronger than that of control group, indicating that the amount of ROS increased. The green fluorescence of PD98059+PDGF-BB group was weaker than that of control and PDGF-BB group, indicating that the amount of ROS decreased. ③ Compared with control group, mitochondrial Cx43 protein expression of PDGF-BB group was significantly increased(P0.01) and mitochondrial Cx43 protein expression of PD98059+PDGF-BB group was significantly PAGE PAGE VI decreased(P0.05). Compared with PDGF-BB group, mitochondrial Cx43 protein expression of PD98059+PDGF-BB group was significantly decreased(P0.01). EC-VSMC Co-culture model: ① MTT assay results showed that the A value of TNF-α group was increased compared with control group(P＜0.05). ② The green fluorescence of TNF-α group was stronger than