不同训练方式对大鼠腓肠肌p53和IL6影响.docVIP

不同训练方式对大鼠腓肠肌p53和IL-6的影响 　　摘 要: 目的:探讨不同训练方式对大鼠腓肠肌p53和IL?6的影响。方法:21只大鼠随机分为3 组,即安静组(C,n=7),耐力训练组(E,n=7),间歇性冲刺训练组(S,n=7)。耐力训 练组和间歇性冲刺训练组进行跑台训练8周;末次训练后24～48 h内,各组大鼠安静状态断 颈处死,取腓肠肌。采用Elisa法分别测定腓肠肌中p53和IL?6水平。 结果:1) E组(P 0.05)和S组(P0.01)腓肠肌p53蛋白表达显著低于C组。2) E,S组腓肠肌IL?6表达与C 组相比无显著性差异。结论:冲刺训练和耐力训练对P53的表达产生下调作用,在一定程度 上可延缓骨骼肌衰老,同时间歇性运动效果更明显;而不同训练方式对IL?6的表达并未见 有显著影响。 　　关键词:运动;大鼠;P53;IL?6;训练方式 　　中图分类号:G804.7 　　文献标识码:A 　　文章编号 :1007-3612(2010)02-0056-03 　　Effects of Different Exercise Modes on P53 and IL?6 Expression 　　of Gastrocnemius Muscle in Rats 　　LIU Jing?xia, QI Zheng?tang, DING Shu?zhe 　　(College of Physical Education and Health, East China Normal Un iversity, Shanghai 200241, China) 　　Abstract: Objective: To investigate the effects of different exercise modes on p 53 and IL?6 in rats’ gastrocnemius muscle. Methods:Twenty?one male SD rats a re 　　randomly and equally divided into three groups: control group(C, n=7),enduran ce exercise group(E, n=7), and intermittent strenuous exercise group(S, n=7). 　　The rats are sampled after 8?week exercise training. Between 24h and 48h after 　　t he last training, the rats are killed, gastrocnemius muscle are excised quickly 　　for ELISA. Results: 1) The P53 expression of gastrocnemius muscle is downregulat ed significantly in E(p0.05) and S(p0.01) group; 2) The IL?6 expres sion of g astrocnemius muscle has no significant change in E and S group. Conclusion: The 　　effects of endurance mode and intermittent strenuous mode exercise on p53 can de lay aging of muscle; But the connection between the expression of IL?6 and diff erent exercise modes is not clear yet. 　　Key words: exercise training; rat; P53; IL?6; exercise mode 　　 　　p53基因是迄今发现与人类肿瘤相关性最高的基因,在短短的十多年里,人们对p53基因 的认识经历了癌蛋白抗原,癌基因到抑癌基因的三个认识转变。p53在多个细胞信号途径中 起介导作用。这些途径最终可导致细胞周期停止、DNA修复、细胞老化、衰老和凋亡。p53蛋 白的DNA结合作用及反式激活作用还提示其参与细胞生长调控,p53监视基因组的完整性,如 有损伤,p53阻止DNA复制,以提供足够的时间使损伤DNA修复;如果修复失败,p53蛋白则引 发细胞凋亡[1]。最近的研究进展表明p53被激活后对线粒体产生一系列调节作用, 包括增加 线粒体DNA的结构稳定性、调节线粒体的呼吸功能,以及调控细胞有氧和无氧代谢路