不同免疫检验方法检测乙肝病毒感染血清标志物对比评价.docVIP

不同免疫检验方法检测乙肝病毒感染血清标志物的对比评价 　　[摘要] 目的 探讨不同免疫检验方法检测乙肝病毒感染血清标志物的效果差异性。方法 方便选择2016年8月―2017年8月间该院收治的80例乙肝病毒感染患者实施观察研究，对所有患者均实施电化学发光法、酶联免疫吸附法检测，分析总结两种免疫检测方式对患者血清标志物（表面抗原HBsAg、表面抗体HBsAb、e抗原HBeAg、e抗体HBeAb、核心抗体HBcAb）检测结果差异性。结果 电化学发光法检测HBsAg、HBeAg、HBeAb阳性率70.0%、31.3%、28.8%较酶联免疫吸附法52.5%、17.5%、15.0%相比明显升高（P=0.023、0.043、0.035）。电化学发光法检测HBsAb、HBcAb阳性率17.5%、72.5%较酶联免疫吸附法15.0%、70.0%相比差异无统计学意义（P=0.668、0.727）。实施电化学发光法对乙肝病毒感染诊断准确率97.5%较酶联免疫吸附法检测准确率83.8%明显较高（P=0.003）。结论 免疫检验方法中电化学发光法检测乙肝病毒感染血清标志物效果优于酶联免疫吸附法。 　　[关键词] 免疫检验；电化学发光法；酶联免疫吸附法；乙肝病毒感染；血清标志物 　　[中图分类号] R5 [文献标识码] A [文章编号] 1674-0742（2017）12（c）-0035-03 　　[Abstract] Objective This paper tries to investigate the difference clinic effect of different immunoassay methods in detecting serum markers of hepatitis B virus infection. Methods 80 patients with hepatitis B virus infection were convenient selected from August 2016 to August 2017 in this department. All patients were examined by electrochemiluminescence and enzyme-linked immunosorbent assay （ELISA）， and the results of two immunoassay methods Patient serum markers （surface antigen HBsAg， surface antibody HBsAb， e-antigen HBeAg， e-antibody HBeAb， core antibody HBcAb） test results differences. Results The positive rates of HBsAg， HBeAg and HBeAb by electrochemiluminescence were 70.0%， 31.3% and 28.8%， respectively， which were significantly higher than those by enzyme-linked immunosorbent assay of 52.5%， 17.5% and 15.0% （P=0.023， 0.043， 0.035）. The positive rate of HBsAb and HBcAb detected by electrochemiluminescence was 17.5%， 72.5% compared with 15.0% and 70.0% of enzyme-linked immunosorbent assay （P=0.668， 0.727）. The diagnostic accuracy of electrochemiluminescence assay for hepatitis B virus infection was 97.5%， which was significantly higher than that of enzyme-linked immunosorbent assay （83.8%） （P=0.003）. Conclusion Immunoassay method in the detection of hepatitis B virus infection by electrochemiluminescence method serum markers better than enzyme-linked immunosorbent ass