不同氮素形态与配比对桔梗生长及桔梗皂苷D含量影响.docVIP

不同氮素形态与配比对桔梗生长及桔梗皂苷D含量的影响 　　[摘要]研究不同形态氮素处理对桔梗生长和桔梗皂苷D含量的影响，为桔梗氮肥合理施用提供借鉴。采用盆栽方式，对桔梗进行不同氮素形态配比处理，测定桔梗生长相关指标，包括生物量、光合参数及NR，GS活性，硝态氮，铵态氮含量和SOD，POD，CAT活性，桔梗皂苷D含量。结果表明：NH4+N/NO3-N为0∶100处理，桔梗叶片NR活性、硝态氮含量、净光合速率及生物量达到最大值。叶片中GS活性在NH4+N/NO3-N为25∶75处理最大，铵态氮含量在NH4+N/NO3-N为75∶25处理为最大值。随着NO3-N比例的增加，SOD活性先降后升，CAT活性先升后降，MDA含量先降后升，且CAT与MDA呈完全相反变化趋势，均在NH4+N/NO3-N为25∶75处理分别达到最大值与最小值。桔梗皂苷D含量也随NO3-N比例增加而呈先升后降的趋势，在NH4+N/NO3-N为25∶75处理达到最大值，较酰胺态氮处理升高了2581%。不同氮素形态及配比对桔梗光和特性、氮素代谢和抗逆调节指标、产量及质量影响显著。NH4+N/NO3-N为25∶75处理较适宜桔梗生长及桔梗皂苷D含量的累积。 　　[关键词]桔梗；氮素；生理；活性成分 　　[Abstract]To providing evidence about nitrogen adequate application of Platycodon grandiflorum， the pot culture experiment was conducted to study the effect of nitrogen on the growth， physiological metabolism and the quality of P grandiflorum The activity of NR，GS and SOD，POD and CAT were determined And the nitrate and ammonium nitrogen content， photosynthetic characteristics， active components of P grandiflorum were determined The results showed that the nitrate nitrogen content and Pn biomass reached its maximum value， when NH4+N/NO3-N was 0∶100， the activity of NRThe activity of GS was the highest at the NH4+N/NO3-N ratio of 25∶75 and ammonium nitrogen content was the highest at 75：25The activity of SOD decreased and then increased with the increasing of NO3-NAt the NH4+N/NO3-N ratio of 25∶75，the activity of CAT had its maximum value and the content of MDA had the minimum value At the same time， the content of platycodon D was the highest at this treatment The studies had shown that different nitrogen forms and ratio had a significant effect on the characteristics of photosynthetic physiology， nitrogen metabolism and resistance adjustment， growth and the quality of P grandiflorumThe NH4+N/NO3-N ratio of 25∶75 was a suitable ratio of nitrogen forms for the growth of P Grandiflorum and accumulating the content of platycodon D 　　[Key words]Platycodon grandiflorum； nitrogen； physiological metabolism； active component 　　桔梗Platycodon g