中介素153对肺缺血再灌注损伤大鼠肺组织PPARγ表达影响.docVIP

中介素1-53对肺缺血再灌注损伤大鼠肺组织PPAR-γ表达的影响 　　[摘要] 目的 观察中介素1-53对大鼠肺缺血再灌注损伤(LIRI)肺组织过氧化物酶增殖物激活受体γ(PPAR-γ)的影响。方法 将健康Wistar 大鼠54 只随机分为手术对照组(C组)、缺血再灌注组(IR组)、中介素干预组(D组)。每组分别在缺血45min、再灌注60min、120 min 3个时点处死6 只大鼠,观察肺组织病理形态变化,观察各组肺湿干质量比值(W/D)、CINC-1含量、PPAR-γmRNA、PPAR-γ蛋白的改变。结果 与IR组比较,中介素1-53预处理能够明显升高PPAR- γmRNA(P0.05),减轻肺水肿,并降低CINC-1蛋白含量(P0.05)。结论 中介素1-53能上调PPAR-γ的表达,可能通过PPAR-γ的抗炎作用对LIRI起保护作用。 　　[关键词] 肺缺血再灌注损伤;中介素1-53;过氧化物酶体增殖物激活-γ;保护作用 　　[中图分类号] R364.1+2;R563 [文献标识码] A [文章编号] 1673-9701(2010)04-03-03 　　 　　Effects of Intermedin1-53 on Pulmonary Peroxisome Proliferator-activated Receptor-γ Expression in Rat Lung Ischemia-reperfusion Injury 　　LIU NiLI JianqiangYANG ShujuanZHAO Hui 　　Department of Respiratory,the Second Affiliated Hospital of Shanxi Medical University,Taiyuan 030001,China 　　 　　[Abstract] Objective To investigate the effect of intremedin 1-53 on peroxisome proliferator-activated receptor-γ(PPAR-γ) in rat lung ischemia-reperfusion injury. Methods Fifty-four healthy Wistar rats were randomly divided into three groups:surgical control group(C group), ischemia-reperfusion group(IR group)and intermedin-pretreated group(D group). At three time points(45 min ischemia,60 min and 120 min reperfusion),6 rats from each group were killed,the lung histopathological changes were observed and the wet /dry weight ratio of lung tissue,CINC-1 content,PPAR-γmRNA and PPAR-γprotein in each group were determined. Results Compared with IR group,the pre-treatment by intermedin1-53 significantly increased PPAR-γmRNA(P0.05),and decreased interstital edema and CINC-1content(P0.05). Conclusion Intermedin1-53 may upregulate PPAR-γexpression,which has protective effects on rat lung ischemia- reperfusion injury. 　　[Key words] Lung ischemia-reperfusion injury;Intermedin1-53;Peroxisome proliferator- activated receptorγ;Protective effects 　　 　　中介素(IMD)是2004年由Roh等[1]用种系特征方法分析基因库,从人类和其他脊椎动物基因组证实的一种新的降钙素(CT)/降钙素基因相关肽(CGRP)家族肽.IMD前体蛋白可被内源性肽酶降解为prepro-IMD95-147即IMD1-53,目前国内外有报道中介素1-53对油酸致急性肺损伤和心肌缺血