大鼠脑出血后AQP―4CRP及VEGF表达与脑水肿相关性研究.docVIP

大鼠脑出血后AQP―4CRP及VEGF表达与脑水肿相关性研究 　　[摘要] 目的 探讨大鼠脑出血后不同时间水通道蛋白-4（AQP-4）、C-反应蛋白（CRP）及血管内皮细胞生长因子（VEGF）的表达及其与脑水肿的相关性。 方法 将90只大鼠分为对照组18只，实验组72只。其中对照组为假出血模型，实验组采用立体定向仪建立大鼠脑出血的模型，在脑出血后观察大鼠神经功能的变化。分别断头取脑，采用ELISA法检测血清CRP、VEGF，免疫组化方法检测脑组织AQP-4。 结果 实验组6 h到14 d 足印迹试验评分高于对照组（P0.05或P0.01）。AQP-4、CRP和VEGF表达在6 h即升高，24 h、48 h、3 d、7 d明显升高，差异有统计学意义（P0.01）。 结论 脑出血后AQP-4、CRP、VEGF呈现过度表达，其动态变化与大鼠脑出血后神经功能反应、脑细胞水肿存在一定联系。推测三者参与了脑出血后脑水肿的形成与消退。 　　[关键词] 脑出血；水通道蛋白-4；C-反应蛋白；血管内皮细胞生长因子；脑水肿 　　[中图分类号] R743.34 [文献标识码] A [文章编号] 1674-4721（2016）04（c）-0125-04 　　Correlation between AQP-4，CRP，VEGF expression and brain edema after cerebral hemorrhage in rats 　　ZHANG Bo1 LIU Jian2 　　1.Department of Neurosurgery，the First People′s Hospital of Guiyang，Guizhou Province，Guiyang 550002，China；2.Department of Neurosurgery，the Affiliated Hospital of Guizhou Medical University，Guizhou Province，Guiyang 550004，China 　　[Abstract] Objective To make discussions over the expressions at different times AQP-4，CRP and VEGF after cerebral hemorrhage of rat as well as the relevance with cerebral edema. Methods 90 rats were divided into two groups： control group （including 18 rats） and experimental group （including 72 rats）.The control group was the model of false bleeding.As to the experimental group，the stereo-tactic techniques were adopted to establish the model of cerebral hemorrhage of rats.After the cerebral hemorrhage，observations were made on the changes of rats′neurological functions at different time.After that，the heads of rats in the two groups were cut to get the brain.The ELISA method was adopted to test the serum CRP and VEGF，and the immunohisto-chemical method was adopted to test the brain tissue AQP-4. Results The foot imprinting test from grade 6 h to 14 d in the experimental group were higher than those in the control group （P0.05 or P0.01）.AQP-4，CRP and VEGF expression increased at 6 h，and increased obviously at 24 h，48 h，3 d and 7 d.Besides，the differences were significant （P0.01）. Conclusion After the c