不同剂量支链氨基酸对运动大鼠下丘脑5-羟色胺及其前体的影响-运动人体科学专业论文.docxVIP

南京体育学院硕士学位论文 南京体育学院硕士学位论文 II II 浆 f-Trp/BCAA 比值随时间延长下降；（5）与对照组相比，小剂量和 中剂量补充组大鼠力竭游泳运动恢复期下丘脑细胞外液 5-HT 峰值出 现时间延迟，且小剂量组 5-HT 浓度随时间变化整体下移，而大剂量 组 5-HT 浓度随时间变化整体上移。 结论：1、持续 6 周补充 BCAA 造成的蛋白质合成增加和脂肪的减少 基本保持动态平衡；2、血氨随着 BCAA 补充剂量的增加而升高，小 剂量补充 BCAA 对机体血氨的生成无影响；3、补充 BCAA 能提高大 鼠力竭游泳运动能力，但并不随 BCAA 补充剂量的增加而提高；4、 补充 BCAA 可能提高了血浆 BCAA 与 f-Trp 竞争通过血脑屏障的能力 且具有推迟和减少脑 5-HT 生成的作用 5、小剂量（1.25g/kg·BW） 补充 BCAA 对提高大鼠运动能力效果最佳。 关键词：支链氨基酸；下丘脑；色氨酸；5-羟色胺；运动性中枢疲劳 Ⅲ Ⅲ Abstract Objective: To observe the effects of BCAA Supplementation on BCAA, f-Trp and 5-HT in hypothalamic extracellular fluid in rats recovering from, exhaustive swimming, to analyze whether BCAA Supplementation can improve the exercise capacity of rats and compare the effect of different doses of BCAA supplement, explore the optimal dose of supplement BCAA. Methods: 32 rats were randomly divided into control group, small-dose supplement group (1.25g/kg body weight), middle supplementary dose group (2.5 g / kg body weight) and high-dose supplement group (5 g / kg body weight), each group including 8 rats. One rat was randomly selected from each group to determine the exhaustive exercise load. BCAA supplementation group was once a day in the morning and BCAA supplement six times a week expected Sunday, lasting supplement for six weeks. After six weeks, the blood was collected from tail vein of rats to test ammonia in the afternoon the day before exhausted swimming. Rats were anesthesia with 20% urethane 1mL/200g weight subcutaneous injection after exhaustive swimming positioning with stereotaxic instrument, the guide tube implanted the right side of the hypothalamus (AP: +0.03 mm, ML: +0.08 mm, DV:-0.67mm) and fixed microdialysis probes along the guide tube is inserted into the right side of hypothalamus, infusion of artificial cerebrospinal fluid with the speed of PAGE PAGE IV 2μl/min to balance 2h; and collecting microdialysis samples 4h, 5h, 6h , 8h and 24h after exercise respectively. By capillary electrophoresis -