不同亚型贲门肠上皮化生与贲门癌关系的研究-肿瘤学专业论文.docxVIP

摘要 摘要 状细胞的纹状缘，Ⅲ型肠化及癌变组织中主要表达在细胞质中，其表达的位点与 病理类型相关(Kappa=0.490)。 结论：1、Ki67 蛋白在贲门炎、肠化组织及癌变组织中表达率逐渐增高，表 明随着组织学形态的改变，细胞的增殖能力逐渐增强。提示贲门肠上皮化生是贲 门癌变过程中一个重要环节。2、DAS-1 蛋白在贲门炎、肠化组织及癌变组织中 的表达差异具有统计学意义，提示该蛋白有可能成为提高贲门癌早期诊断率的监 测指标及预警分子。3、伴 DAS-1 过表达的Ⅲ型贲门肠化与贲门癌的关系密切， 可能是河南贲门癌高发区 GCA 的一种重要的癌前病变。4、TFF3 蛋白在贲门炎 及Ⅰ型肠化组织中主要表达在柱状细胞的纹状缘，Ⅲ型肠化及癌变组织中主要表 达在细胞质中，其表达位点的转变可能说明了该蛋白表现为抑癌和促癌两种不同 作用的结构基础。 关 键 词：贲门癌，肠上皮化生，Ki67，DAS-1，TFF3，免疫组织化学 论文类型：基础研究 II Subject: Relationship between Subtypes of Cardia Intestinal Metaplasia and Gastric Cardia Adenocarcinoma Specialty: Oncology Name: Hao Shuguang Supervisor: Professor Feng Xiaoshan ABSTRACT Objective: To identify the relationship between subtypes of cardia intestinal metaplasia and gastric cardia adenocarcinoma by analyzing the expression of Ki67, das-1 and TFF3 proteins in subtypes of cardia intestinal metaplasia and cardia lesions at all levels. Methods: One hundred and seventy cases of cardia tissues by gastroscope and carida cancer tissues by surgery were collected in Linzhou area of Henan province. immunohistochemical S-P method has been employed to examine the expression of Ki67, das-1 and TFF3 proteins in 25 gastric cardia hilitis, 15 intestinal metaplasia without cancer, 65 intestinal metaplasia adjacent cancer 65 gastric cardia adenocarcinoma issue specimens. Eighty intestinal metaplasia issue specimens were typed by the Mucus staining method. The relationship between subtypes of cardia intestinal metaplasia and gastric cardia adenocarcinoma was analyzed by SPSS soft. Results: The positive rates of Ki67 protein in gastric cardia hilitis, intestinal metaplasia, and carcinoma tissues were 16.0%, 43.8%, and 53.8%, respectively. Statistical analysis using chi-square showed the expression level of Ki67 protein was different and correlated well with the pathological grading of the lesions (r=0.436, P0.05). Eighty cases of gastric cardia intestinal metaplasia were classified three types by mucosal staining. The proportion o