不同抗螨性能东方蜜蜂obp3基因的克隆与表达变化规律的分析-动物遗传育种与繁殖专业论文.docxVIP

I II otblnr-et se w 分析可见兔抗蜜3P蜂BO 抗3P血B清O- 与T pE 抗原发生特异性结合，表 明 obp3 基因已i o在l.cE 中获得表达。东方蜜蜂 obp3 基因多克隆抗体制备成功。 关键词：东方蜜蜂；obp3 基因；序列分析；生物信息学分析；原核表达载体；多克 隆抗体 PAGE PAGE III t s b A t cra sTyhaissa nisgi mi a nig ytlza na e nechrteff di of ehte heowl nc qeuse of obp3 nat  (odonrg ndbii ni e otpr  neenatwt3ts)i sbeer- aorr  vanatsi ser-  orar anvad non-  s piA arneac , ngnhadcir aes  lofar  stpiomret  lnaadit npoisasse  xepr  nrsett  pnaee w-t be  hteo wyt  hone , se  bpelmas  ot pnidfhi  ohintesal er  nee wt be  obp3nad e hn-atcttsiasretr-  ofraor va ni y yl,l hnoian.eFe be  l htoelnaypcol  biot dnya  t nisa gnat nabi  ocmre  3P BO  sdaerwpea pr ot de ovpilrairet a m  r ohfrte ufrhc.r aeser sr e m1i.r P  erde wginse dengdiocrca  AoNtsDec nc  qeuse  f o obp3 soafrpeiAfill e m  . L ni I BCN  hse.ATNDc  sofpiA arneac  er e wn  kaetset a spalmet  yrort- ac  t ounegneg olnci ot ni a  obt  ehte heowlnc  qeuse  of oberp3h. eTdetsi xe  e- eehtrnes  ons  oint a utm  s ogf/ a  , a/, g nad  a/ g  nad oensenesoint a utm  ) F/ oL(ft/ c  t a 164bp,200bp,341bp nad 192 olc i yl viet c  pseer  ni e onbcp3qenusatet si ser-  oorarmrf  va on-  senatbsieser-nadorar va I g/( ,a) L/I(c/  .ase be ) S/ Q(t/  ,c) V/  Mg/( ,a  ()c/N/,t) D(V/aL,g)/(/D,/c) NR/ gK/(),ga/( ,a) S/ T g/( ,c) T/ V( a/ ,g ) V/ L  )dSn/ aR( c/ a  er edewt cet  denee wt be  s piA arneacuisci  bar F  nadsarpefiAil-l e m t a 59,161,201,217, 231,234,235,318,321,348,364,367,372,374  nad 452bp  olc i yl.Aviet c  pseer  nle Gnec -bas  sdaetwcet de  nsiarpeiAfill e mnigr  pa ocm  hti  ws piA arneac edcune-oet bas  evioefuhtctre  ocns  ses ba c、 a、5g3 51-  bpodlecl .ai Ivtesr e  nc qeuse r eff  dsei  necr  of o.bspei3cni pse RCP-2.TR  tdset  hsow  t htaer  hte  sa wnohigsi she  xepr  ne ni badom  naddaoefh  obp3f o nigtsi ser-  hoterar va  , e.lsehibenawe M  er htenatsciaf wnigiis oef  necr eff di  n