赤霞珠葡萄黄化突变体生理特性及相关基因研究-studies on physiological characteristics and related genes of cabernet sauvignon grape yellow mutant.docxVIP

赤霞珠葡萄黄化突变体生理特性及相关基因分析 赤霞珠葡萄黄化突变体生理特性及相关基因分析 4 4 The mutant is heterotrophic, the leaves of the mutant had a greater ability to absorb and impress the 15N, 1.3 times of the green branches. 15N tracer test indicated that the ability of the mutant fruit was 83% of the green branches. The etiolation branches satisfied itself by long-distance transport. The mutant branches was thin and delicate, high proportion of heart centre, which resulted in defoliation of leaf in advance, winter disorder and finally died. The fruits of the Cabernet Sauvignon Albino Mutant had smaller fruit ear, lighter weight, comparing with the control; the contents of the soluble solid, sugar and proteincwas lower than those in the control and were only 66.67%, 60.67% and 47.14% of the control. The titrable acidity was 1.92 time of the control. Through HPLC, we found that in the mutant fruits, fruit sugarfruit and glucose were only 65.37% and 65.78% of the control, respectively. Sucrose doesn’t change much. The tartaric acid is 76.71% of the control; while the content of oxalic acid, malic acid, malic acid, acetic acid and citric acid was 3.69, 4.26, 2.71, 2.20 and 1.84 times of the control respectively; however, the succinic acid content didn’t change much. The fruits of Cabernet Sauvignon Albino Mutant had a light color. And the anthocyanin decreased a lot compares with control, which might be related with PAL activity, while phenol was rich; the etiolation leaves had a lower content of flavonoid and phenol. The PPO activity was much high. Using gene chip, we detected the gene expression profile of the leaves and fruit in the mutant. Under the situation of P-value0.01, we got 2911 and 775 genes which changed significantly in the leaves and fruits, respectively. 184 genes changed both in the leaves and fruits. After functional classification, the significantly changed genes in the mutant leaves included the ones in synthesis of plant hormones and proteins, carbon metabolism of photosynthetic orga