重组腺病毒介导bmp-2bfgf体外诱导山羊bmsc成骨分化的研究-recombinant adenovirus mediated bmp - 2 bfgf induces osteogenic differentiation of goat bmsc in vitro.docxVIP

广州医学院硕士学位论文重组 广州医学院硕士学位论文 重组腺病毒介导 BMP-2/bFGF 体外诱导山羊 BMSC 成骨分化的研究 PAGE PAGE 5 Osteogenic differentiation of goat bone marrow mesenchymal stem cells induced by adenovirus-mediated BMP-2/bFGF in vitro Graduate：Xu Hui-Hua Tutor：Guo Qi-Feng Abstract Background: It is undoubted that bone marrow mesenchymal stem cells (BMSCs) are the ideal seed cells for tissue engineering bone. A variety of cytokines are involved in the repair of bone injury, such as bone morphogenetic protein 2 (BMP-2) in transforming growth factor (TGF-β) superfamily, the most accurate member inducing bone formation, as well as basic fibroblast growth factor (bFGF) , a mitosis sources with broad spectrum, which can promote the formation of cartilage and bone tissue, but also directly stimulate angiogenesis to improve the new bone formation of implanted tissue engineering bone , and can also induce bone occurrence with BMP-2 and play an important role in bone healing process. The osteogenic activity of BMSCs induced by BMP-2 and bFGF together is greater than bFGF or BMP2 alone. However, exogenous bone induction factors easily lose activity through protease breakdown and are dispersed and diluted, with short continuous effect time and low efficiency, and can not play continuous local stimulation and bone induction effect. Therefore, gene therapy inducing bone formation through transgene can overcome the lack of direct application of exogenous growth factors.The choice of a suitable vector carrying the target gene is the key to gene therapy. Adenovirus is one of the most widely used gene vector efficiently into cells. It is a non-pathogenic replication-defective vector and can provide long-term gene expression with very low immunogenicity. It has wide host range and can infect humans, mice and other genera, with a range of organizations affinity. It exists as an additional body form after infecting host, and the genes are not integrated into the host cell genome, without the danger of insertion mutation