浅议中医虚证与食管癌Cmyc基因表达关系研究.docVIP

浅议中医虚证与食管癌Cmyc基因表达关系研究 　　【摘要】目的:研究中医虚证与食管癌C-myc基因表达的关系，探讨其临床意义。方法:根据中医辨证标准，将50例患者分为食管癌伴有虚证和不伴有虚证两组。胃镜和手术标本，进行病理学检查及C-myc的免疫组化检查，C-myc基因表达结果用图像分析进行半定量测定。结果:50例患者中有21例（42%）患者有不同程度的虚证表现，其中气虚8例，阴虚5例，气阴两虚4例，气血两虚3例，阴阳两虚1例。食管癌伴有虚证组患者比不伴有虚证组患者C-myc的表达增强，其差异具有统计学意义（p0.01）,虚症患者的转移发生率明显高于不伴虚证者（p0.05）。结论:中医虚证与食管癌C-myc基因表达关系密切。虚症患者更易发生浸润和转移。 　　【关键词】食管癌；虚证；C-myc 　　Study on relationship between deficiency syndrome of TCM and the C-myc gene expression in patients with esophageal carcinoma 　　Zhang Rongguang 　　【Abstract】ObjectTo explore the relationship between deficiency syndrome of TCM and the C-myc gene expression in patients with esophageal carcinoma . MethodsAccording to syndrome differentiation of TCM, 50 patients of esophageal carcinoma were evaluated and divided into two groups, the deficiency syndrome group (DS group) and non- deficiency syndrome group (NDS group). Tissue C-myc gene expression was examined by immunohistochemistry.ResultsTwenty one cases (42%) in the 50 patients were DS. Eight patients were Qi-deficiency , 5 Yin-deficiency , 4 Qi -Yin deficiency , 3 Qi-and blood deficiency , 1 Yin-Yang deficiency. The C-myc expression in DS group was higher than that in NDS group. There was significant differences between the two group (p0.01). In DS group , the metastatic rate was higher too. Conclusion Deficiency syndrome of TCM is closely correlated with the C-myc gene expression in patients with esophageal carcinoma. 　　【Key words】esophageal carcinoma;deficiency syndrome;C-myc 　　【中图分类号】R350 【文献标识码】B【文章编号】1005-0515(2011)08-0043-02 　　 　　中医学理论认为虚证是肿瘤发生和发展的重要因素之一，许多现代医学实验和临床研究也表明中医虚证与恶性肿瘤的发生有着密切的联系。本文探讨中医虚证与食管癌组织C-myc基因表达的关系及其意义。 　　1 对象与方法 　　1.1 病人50例，男性38例，女12例。年龄45~77岁，平均55.8岁。根据中医辨证标准，将50例患者分为食管癌伴有虚证和不伴有虚证两组［1］。食管癌病变分段及分期标准采用UICC于1987年公布的食管癌病变部位分段标准［2］。 　　1.2 免疫组化方法: 　　胃镜或手术标本切4μm石蜡切片，进行病理学检查和C-myc免疫组化ABC法染色。一抗为博士德公司产品，ABC试剂盒为Zymed公司产品。C-myc的免疫组化方法参加文献［3］。C-myc基因表达结果用图像分析进行半定量测定，测定阳性表达面积占总面积的百分比，结果以χ±s表示。 　　1.3 统计学处理: 　　采用SPSS13.0软件包进行t检验。 　　2 结果 　　2.1 C-m