标本不同保存时间及温度对血液筛查结果影响研究.docVIP

标本不同保存时间及温度对血液筛查结果影响研究 　　[摘要] 目的 探讨标本不同的保存时间及温度对血液筛查结果的影响。 方法 对53例患者的静脉血进行采集，对采集时间进行准确记录并编号，放置半小时后离心。一份置于4℃冰箱之中分别保存0、6、12及24 h后对总蛋白、清蛋白、葡萄糖、尿酸、ALT及K+进行检测；另外一份于室温条件下保存0、6、12及24 h后对上述指标进行检测。采用罗氏MODULAR P800全自动生化检测仪对上述指标进行检测。 结果 ①4℃下，6～24 h总蛋白水平均显著高于0 h（P0.05）；6～24 h葡萄糖水平均显著高于0 h（P0.05）；0～24 h尿酸水平差异无统计学意义（P0.05）；12 h及24 h K+水平显著高于0 h、6 h，0、6 h K+水平差异无统计学意义（P0.05）；②室温条件下，0、6、12、24 h总蛋白、清蛋白、尿酸及葡萄糖水平差异均无统计学意义（P0.05）；6、12、24 h ALT及K+水平均显著高于0 h（P0.05）。 结论 医院实验室应对血液标本的保存时间及保存温度进行规范，以确保血液筛查结果的准确度。 　　[关键词] 血液标本；保存时间；保存温度；血液筛查 　　[中图分类号] R446.11 [文献标识码] B [文章编号] 1673-9701（2015）12-0096-03 　　Effects of different specimens save time and storage temperature of blood screening results 　　YAN Feng 　　Department of Clinical Laboratory， the Center Blood Station of Yantai City in Shandong Province， Yantai 264003， China 　　[Abstract] Objective To investigate the effects of different specimen storage time and storage temperature of blood screening results. Methods A total of 53 patients of blood were collected， accuratly recorded of the acquisition time and number， centrifugation after placed half an hour. Total protein， albumin， glucose， uric acid， ALT and K+ were detected being placed in a refrigerator at 4 ℃ were preserved after 0 h， 6 h， 12 h and 24 h； In addition these indicators were detected at a stored at room temperature after 0 h， 6 h， 12 h and 24 h. The indicators were detected by using Roche MODULAR P800 automatic biochemical analyzer. Results （1） At 4℃， 6～24 h total protein levels were significantly higher than 0 h（P0.05）； 6～24 h glucose levels were significantly higher than 0 h （P0.05）； 0～24 h uric acid levels were not significantly different （P0.05）； 12 h and 24 h K+ levels were significantly higher than 0 h， 6 h， and 0 h， 6 h K+ levels were not statistically different （P0.05）； （2） At room temperature， 0 h， 6 h， 12 h， 24 h total protein， albumin， uric acid and glucose levels were not significantly different （P0.05）； 6 h， 12 h， 24 h ALT and K+ levels were significantly h