靶向沉默RACK1基因对肺腺癌裸鼠移植瘤生长抑制作用.docVIP

靶向沉默RACK1基因对肺腺癌裸鼠移植瘤生长抑制作用 　　【摘要】 目的：研究沉默RACK1基因对人肺腺癌细胞株A549裸鼠皮下移植瘤生长的影响。方法：使用人肺腺癌细胞株A549建立裸鼠皮下移植瘤模型。待肿瘤直径为0.3～0.5 cm时将荷瘤裸鼠随机分成3组，每组6只，分别于瘤内注射生理盐水（空白组）、空载质粒-脂质体复合物（空载组）及shRNA-RACK1质粒-脂质体复合物（实验组）。观察三组裸鼠的皮下移植瘤体积变化，绘制肿瘤生长曲线，并用Western blot检测各组肿瘤组织中RACK1蛋白的表达。结果：移植瘤形成后第28天，与空白组和空载组比较，实验组移植瘤生长速率、肿瘤体积和重量均明显降低（P0.05）。转染干扰载体RACK1-shRNA对裸鼠肺腺癌移植瘤的的抑瘤率为39%。Western blot结果显示，实验组移植瘤组织中RACK1蛋白的表达明显低于空白组和空载组，差异均有统计学意义（P0.05）。结论：沉默RACK1基因对肺腺癌裸鼠移植瘤生长有明显抑制作用。 　　【关键词】 肺腺癌； RACK1基因； 裸鼠 　　Inhibitory Effect of Targeted Silencing RACK1 Gene on the Growth of Lung Adenocarcinoma Xenograft in Nude Mice/MI Xiao-fang，GAO Xiao-yun，LIANG Gang，et al.//Medical Innovation of China，2016，13（35）：024-027 　　【Abstract】 Objective：To study the effect of silencing RACK1 gene on the growth of human lung adenocarcinoma cell line A549 in nude mice.Method：BALB/C nude mice were subcutaneously transplanted with lung adenocarcinoma cell lines A549 and tumor bearing nude mice were randomly divided into 3 groups when the tumor diameter was 0.3-0.5 cm，and 6 rats in each group，they were injected with normal saline，empty plasmid liposome complex and shRNA plasmid liposome complex.The tumor volume changes of the three groups were observed，the tumor volume of nude mice were recorded and the expression of RACK1 protein was detected by Western blot.Result：28 days after tumor formation，there were obvious decreases in tumor growth rate，tumor mass，as well as tumor weight in transfected group，comparing with empty vector transfeeted group and untransfected group（P0.05）.Transfection of RNA interference can inhibit the growth of xenograft tumor by 39%.Western blot revealed that the expression of RACK1 protein of transfected group were significantly lower than those in untransfected group and empty vector transfected group（P0.05）.Conclusion： Silencing RACK1 gene can effectively inhibit the growth of lung adenocarcinoma in nude mice. 　　【Key words】 Lung adenocarcinoma； RACK1 gene； Nude mice 　　First-author’s address：Shanxi Medic