人参锈腐病生防细菌的分离筛选与鉴定-吉林农业大学学报.PDFVIP

吉林农业大学学报 　2010 ,32 (2) :136～139 ,144 http :/ / x uebao. j lau . edu . cn J ournal of J ilin A gricultural University Email :j lndxb @vip . sina . com 人参锈腐病生防细菌的分离筛选与鉴定 傅俊范, 史会岩, 周如军, 严雪瑞, 石建华 沈阳农业大学植物保护学院 , 沈阳 110161 摘 　要 : 从吉林和辽宁两省不同人参种植区土壤中分离出 836 株细菌 ,从中筛选出 10 株对人参锈腐病菌 ( Cy lindrocarp on destructans) 具有明显抑制作用的拮抗菌株 。选择抑菌效果最好的菌株 BS015 (抑菌圈直径 228 mm) ,对人参几种常见病原菌进行抑菌谱试验 。结果表明:菌株BS015 对人参根腐病菌 ( Fusarium solani) 、人参 ( ) ( ) ( 菌核病菌 Sclerotinia schinseng 、人参黑斑病菌 A lternaria p anax 均有较好的抑菌效果 ,对人参立枯病菌 Rhizoc tonia solani) 抑制效果不明显 。通过对其形态特征 、生理生化及 16S rDNA 序列分析研究 ,菌株 BS015 为枯草芽 ( ) 孢杆菌 B acillus subtilis 。 关键词 : 人参锈腐病 ; 拮抗细菌 ; 筛选 ; 鉴定 ( ) 中图分类号 : S435675 　　　文献标识码 : A 　　　文章编号 : 2010 Isolation Screening and Identif ication of Antagonistic Bacteria against Ginseng Rusted Root Disease FU J unfan , SHI Huiyan , ZHOU Ruj un , YAN Xuerui , SHI J ianhua College of Plant Protection , S heny ang A gricultural University , S heny ang 110161, China Ab stract : Totally 836 bacterial strains were isolated from Ginseng rhizosphere soil of Jilin and Liaoning provinces. Among those bacteria , 10 strains showed strong antibiosis activity to Cy lindrocarp on destruc tans . Isolate BS015 showed a wide range of antagonism against pathogens , such as Fusarium solani , Sclerotinia Schinseng , A lternaria p anax . Based on the phenotypic characteristics and analysis of the 16S rDNA fragment ,BS015 was identified as B acillus subtilis . Key word s : C