铁皮石斛花多糖相对分子质量及其单糖组成研究.docVIP

铁皮石斛花多糖相对分子质量及其单糖组成研究 　　[摘要]为了明确铁皮石斛花中多糖相对分子质量分布及其单糖组成，该研究采用高效凝胶色谱和柱前衍生UPLC对11个铁皮石斛花样品的多糖进行了相对分子质量及其单糖组成分析，并利用SPSS 190软件根据组成的单糖峰面积进行聚类分析，结果表明11个杂交家系铁皮石斛花的粗多糖均分离为三部分（DOP1，DOP2和DOP3），其平均相对分子质量分别为553×105，349×105和212×105。铁皮石斛花多糖主要由葡萄糖、甘露糖、半乳糖、半乳糖醛酸和阿拉伯糖5种单糖组成，其中甘露糖组成比例最高，其与葡萄糖比值为0302～3335；不同家系样品多糖中各单糖的相对含量存在一定差异，11个家系按单糖组成及相对含量划分为4类，该研究基本明确了铁皮石斛花中多糖相对分子质量分布及单糖构成，为其资源利用打下基础。 　　[关键词]铁皮石斛花； 多糖； 相对分子质量分布； 单糖组成； 高效凝胶色谱； 超高效液相 　　[Abstract]In order to provide new information on polysaccharide in Dendrobium officinale flowers，the monosaccharide composition and relative molecular mass distribution of 11 families of flowers were investigated and analyzed by high performance gel filtration chromatography （HPGFC） and precolumn derivatization ultra performance liquid chromatography （UPLC） in this study Then cluster analysis was carried out for the monosaccharide peak areas by utilizing SPSS 190 software The results showed that the polysaccharides of all the above 11 hybrid families of D officinale flower were separated into three fractions （DOP1， DOP2 and DOP3） with the average relative molecular mass of 553×105， 349×105 and 212×105 The polysaccharides in 11 different families were mainly composed of glucose， mannose， galactose， galacturonic acid and arabinose； mannose had the highest proportion among them， with mannose/glucose ratio ofAdditionally， the relative contents of various monosaccharides in different families varied 11 families of D officinale flower could be classified into four categories according to their monosaccharide components and relative contents In this study， the relative molecular mass distribution and monosaccharide composition of polysaccharides in D officinale flowers were defined， which can provide foundations for its resource utilization. 　　[Key words]Dendrobium officinale flower； polysaccharide； relative molecular mass distribution； monosaccharide composition； HPGFC； UPLC 　　铁皮石斛Dendrobium officinale Kiumra et Migo属兰科石斛属Dendrobium多年生附生草本植物