KCNQ1及SLC30A8基因多态性和2型糖尿病患者相关性研究.docVIP

KCNQ1及SLC30A8基因多态性和2型糖尿病患者相关性研究 　　[摘要] 目的 分析KCNQ1及SLC30A8基因多?B性与2型糖尿病（T2DM）患者的相关性。 方法 选取2015年9月～2017年9月间在本院确诊并接受治疗的T2DM患者100例作为观察组，选取同期在本院进行体检健康者100例作为对照组，收集两组对象早晨空腹静脉血5 mL，全自动生化分析仪检测糖化血红蛋白、空腹血糖水平、胰岛素和血脂的水平，基因型检测使用单碱基延伸法，分析不同基因位点和T2DM相关性状况。 结果 SLC30A8基因对照组TT、CT及CC基因型的频率依次为16%、52%及32%，观察组依次为13%、49%、38%，差异无统计学意义（χ2=1.290，P0.05）；对照组T、C等位的基因频率依次为61%、39%，观察组依次为73%、27%，差异有统计学意义（95%CI=0.795～1.684，OR=1.205，χ2=11.553，P0.05）；对照组T、C等位的基因频率依次为53%、47%，观察组依次为64%、36%，差异有统计学意义（95%CI=0.863-2.104，OR=1.171，χ2=12.995，P0.05），CC基因型血清TC、TG、FPG及HbA1c低于CT、TT基因型，血清FINS高于CT、TT基因型，差异均有统计学意义（P0.05）。 结论 SLC30A8基因多态性r点和KCNQ1基因多态性rs2237892位点和T2DM发病及血管并发症有一定相关性。 　　[关键词] 2型糖尿病；SLC30A8基因；KCNQ1基因；相关性 　　[中图分类号] R587.1 [文献标识码] A [文章编号] 1673-9701（2018）09-0001-04 　　Correlation study between gene polymorphism of KCNQ1 and SLC30A8 and type 2 diabetes mellitus 　　XU Ting PAN Bailing DING Pingying 　　Department of Endocrinology， Taizhou Hospital in Zhejiang Province， Linhai 317000， China 　　[Abstract] Objective To analyze the correlation between KCNQ1 and SLC30A8 gene polymorphisms and type 2 diabetes mellitus（T2DM）. Methods 100 patients receiving treatment and diagnosed as T2DM in our hospital from September 2015 to September 2017 were selected as the observation group， and 100 healthy subjects undergoing physical examination in our hospital were selected as the control group during the same period. 5 mL of fasting venous blood were collected from participants of two groups， and detection of glycosylated hemoglobin， fasting blood glucose， insulin and lipid levels were analyzed by automatic biochemical analyzer. Genotype were detected by single base extension method. The correlation between different gene loci and T2DM was analyzed. Results The gene frequencies of TT， CT and CC genotypes of SLC30A8 were 16%， 52% and 32% in the control group， and were 13%， 49% and 38% in the observation group， respectively， with no significant difference（χ2=1.290， P0.05）. The frequencies of T and C alleles were