人脑胶质瘤GDNF基因启动子Ⅰ区H3组蛋白乙酰化修饰情况的研究.docVIP

人脑胶质瘤GDNF基因启动子Ⅰ区H3组蛋白乙酰化修饰情况的研究 　　[摘要] 目的 分析GDNF基因启动子在人体胶质瘤Ⅰ区区间的H3组蛋白乙酰化（acetylization）修饰情况以及人体胶质瘤的GDNF基因启动子表达方式。 方法 选取正常脑组织20例，实施反转录（RT-PCR）的检测方法来检测GDNF基因启动子的相关表达方式，在实时定量荧光PCR（Real-time PCR）的基础上建立好染色质免疫沉淀（CHIP）。 选取人体胶质瘤20例，实施反转录的检测方法来检测GDNF基因启动子的相关表达方式，在此基础上建立好CHIP方法分析GDNF基因启动子在Ⅰ区区间的H3组蛋白乙酰化的相关修饰情况。 结果 实时定量荧光PCR方法在检查人体胶质瘤GDNF基因启动子方式的表达方面，随着RT-PCR的检测水平级别升高而升高，转录水平中的正常组以及高低级别组之间差异有统计学意义（P0.05）。 结论 GDNF基因启动子在人体胶质瘤Ⅰ区区间的H3组蛋白乙酰化修饰情况很有可能会影响到GDNF基因的相关表达方式。 　　[关键词] 人体胶质瘤；GDNF；基因启动子；蛋白乙酰化；修饰情况 　　[中图分类号] R739.4 [文献标识码] A [文章编号] 1673-9701（2015）35-0001-04 　　[Abstract] Objective To analyze the H3 protein histone acetylation modification conditions in I region of human gliomas GDNF gene promoter and to explore the expression of human glioma GDNF gene promoter. Methods A total of 20 cases of patients with normal brain tissue were chosen， and the detection method of reverse transcription （RT-PCR） was used to detect the associated expression method of GDNF gene promoter. On the basis of real-time quantitative fluorescent PCR （Real-time PCR）， Chromatin immunoprecipitation （CHIP） was established. 20 cases of patients with human glioma were chosen， and reverse transcription method was implemented to detect the relevant expression of GDNF gene promoter. On this basis， CHIP method was established to analyze the H3 protein histone acetylation modification conditions in I region of human gliomas GDNF gene promoter. Results By real-time quantitative fluorescent PCR method in checking human glioma GDNF gene expression， with the elevated detected levels of RT-PCR， the expression amount increased. There were statistically significant differences in comparing the normal-level transcription group， the low-level transcription group and the high-level transcription group （P0.05）. Conclusion H3 protein histone acetylation modification conditions in I region of human gliomas GDNF gene promoter is likely to affect the related expression of GDNF-related genes. 　　[Key words] Human glioma； GDNF；