玉米SSR连锁图谱构建及叶面积的QTL定位-生物通.PDF

HEREDITAS (Beijing) 2010 6 , 32(6): 625―631 ISSN 0253-9772 研究报告 DOI: 10.3724/SP.J.1005.2010.00625 玉米SSR 连锁图谱构建及叶面积的QTL 定位 刘建超, 褚群, 蔡红光, 米国华, 陈范骏 , , -, 100193 叶片是玉米进行光合作用的主要器官, 叶面积的大小(尤其是穗三叶面积)对于玉米干物质的积累及产量 形成起着至关重要的作用。研究玉米叶面积的遗传基础对于指导玉米高产育种具有理论意义。文章以两个叶面 积差异显著的亲本478× 武312 为基础材料所构建的218 个F 代的重组自交系为作图群体, 构建了一张包含 184 8 个SSR标记的遗传连锁图谱, 图谱总长度为2 084.1 cM, 平均图距为 11.3 cM。通过两年的田间试验对玉米叶面 积(穗三叶)进行了QTL定位分析。两年共定位到7 个和叶面积相关的QTL位点, 2006 年定位到4 个QTL位点; 2007 年定位到3 个QTL位点。在第2 染色体umc1542-umc1518 标记区间发现一个主效QTL位点, 该位点可以在两年 同时检测到, 两年分别解释 12.5%和 17.3%的表型变异。该位点能稳定地检测到且具有较大的贡献率, 可能会 在玉米叶面积分子标记辅助选择上有所应用。 玉米; 叶面积; 微卫星标记; 数量性状基因 SSR linkage map construction and QTL mapping for leaf area in maize LIU Jian-Chao, CHU Qun, CAI Hong-Guang, MI Guo-Hua, CHEN Fan-Jun Key Laboratory of Plant Nutrition , Ministry of Agriculture/Key Laboratory of Plant-Soil Interactions , College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China Abstract: Maize (Zea mays L.) leaf is the main organ for photosynthesis. The area of leaves (especially the ear-leaf and the two leaves above and below the ear-leaf) plays a vital role in dry matter accumulation and grain yield. Therefore, ge- netic information on leaf area has a theoretical significance for breeding maize with high yield. In this study, a genetic link- age map composing of 184 simple sequence repeat (SSR) markers was constructed based on an F8 recombinant inbred line (RIL) population, which was derived from a cross between 478 and W312. The parents showed a significant difference in leaf area. The map covers