‘鲁红一号’元宝枫叶片总RNA提取方法的比较研究.docVIP

‘鲁红一号’元宝枫叶片总RNA提取方法的比较研究 　　摘要：‘鲁红一号’元宝枫叶片中富含大量多糖、多酚物质，严重影响了总RNA提取的产率和质量。为了找出适宜‘鲁红一号’元宝枫叶片总RNA提取的方法，本试验运用试剂盒法、TRIZOL法和改良CTAB法提取其秋季叶片总RNA。结果表明，试剂盒法提取的叶片总RNA OD260/OD280、OD260/OD230均在1.8以上，但产率一般，有DNA污染；TRIZOL法提取的总RNA OD260/OD280、OD260/OD230均在1.5左右，且产率最低；改良CTAB法提取的总RNA OD260/OD280、OD260/OD230均在2.0左右，且产率最高，电泳图较清晰。说明采用改良CTAB法提取的叶片总RNA质量和产率高，可以满足分子生物学进一步研究的需求，是‘鲁红一号’元宝枫叶片总RNA提取的适宜方法。 　　关键词：‘鲁红一号’元宝枫；总RNA提取；改良CTAB法 　　中图分类号：S792.35文献标识号：A文章编号：1001-4942（2015）03-0005-04 　　Comparative Study on Extraction Methods of Total RNA 　　from Leaves of Acer Truncatum ‘Luhong No. 1’ 　　Hao Xueying，Feng zhen，Lü Chuanqing 　　（College of Forestry，Shandong Agricultural University，Taian 271018，China） 　　AbstractA large amount of polysaccharides and polyphenols in Acer truncatum ‘Luhong No. 1’leaves have seriously affected the yield and quality of total RNA extraction.In order to obtain the appropriate method of total RNA extraction，3 methods，Kit method，TRIZOL and modified CTAB，were chosen to extract them from Acer truncatum ‘Luhong No. 1’autumn leaves.The results showed that the total RNA extracted by Kit method had the general yield，the ratios of OD260/OD280 and OD260/OD230 were both above 1.8，and had the pollution of DNA. That extracted by TRIZOL method had the lowest yield，the ratios of OD260/OD280 and OD260/OD230 were both about 1.5. That extracted by modified CTAB method showed clear electrophoresis pattern and highest yield，and the ratios of OD260/OD280 and OD260/OD230 were both about 2.0.The results demonstrated that the yield and purity of the RNA obtained by the modified CTAB method could meet the demands of molecular biology experiment.The modified CTAB method was the appropriate method for total RNA extraction from Acer truncatum ‘Luhong No. 1’ leaves. 　　Key wordsAcer truncatum ‘Luhong No. 1’；Total RNA extraction；Modified CTAB method 　　元宝枫冠大荫浓、树姿优美、叶形美丽，在堤岸、湖边、草地及建筑附近配植皆很雅致，是北方重要的秋色叶树种。但在华北作庭荫树和行道树时，多数表现一般，本课题组经多年研究选育出的‘鲁红一号’元宝枫，在拥有元宝枫观赏特性的基础上秋叶鲜红，红叶持续时间长，观赏价值高。 　　近几年来，关于元宝枫