响应面分析法优化石香薷总黄酮提取工艺的研究.docVIP

响应面分析法优化石香薷总黄酮提取工艺的研究 　　 摘 要：目的：确定石香薷总黄酮的最佳提取工艺条件。方法：以乙醇回流提取法提取石香薷总黄酮。采用分光光度法测定总黄酮的含量，以总黄酮提取得率为考察指标，考察溶媒浓度、倍数、提取时间、提取次数等因素对石香薷总黄酮提取得率的影响，利用响应面分析法优化提取工艺，确定总黄酮的最佳提取工艺。结果：石香薷总黄酮的最佳提取工艺为：加药材33倍量的60%乙醇，于90℃水浴回流提取1次，每次2.2h。此工艺下小鱼仙草总黄酮的提取得率达37.85 mg/g。结论：该工艺提取得率高、操作简便，易于工业化大生产。 　　 关键词：石香薷；总黄酮；响应面分析法 　　中图分类号：R284.1 文献标识码：A 文章编号：1673-7717(2012)01-0116-02 　　Optimisation for Flavonoids Extraction from Mosla chinensis Maxim.By Response Surface Method 　　ZAHNG Qi1,2,HAO Yun?yun1, YU Chen?huan1, WU Qiao?feng1 　　(1. Zhejiang Chinese Medical University, Hangzhou 310053,Zhejiang,China; 　　2.Zhejiang Chinese Medical Hospital, Hangzhou 310018,Zhejiang,China) 　　Abstract:Objective:To optimize the extraction conditions of flavonoids from Mosla chinensis Maxim. Method:Solvent quantity, extraction time and times of extraction as investigation factors had been raised to extract the total flavonoids determined by the method of spectrophotometry. The procedure for the extraction was performed with the extraction yield of total flavonoids as the response. Response surface method (RSM) was applied to determine the optimum extraction process. Result:The optimum extraction condition was as follows: concentration of alcohol was 60%, extraction 1 time, 2.2 hours each time, ratio of liquid to solid 33 at 90℃. The extraction rate of flavonoids under this condition was 37.85 mg/g. Conclution:The method to extract flavonoids from Mosla chinensis Maxim is simple and feasible. It can provide the scientific basis for further development of Mosla chinensis Maxim. 　　Key words:Mosla chinensis Maxim.；flavonoid；RSM 　　 　　 石香薷Mosla chinensis Maxim.为唇形科植物石香薷的全草，又名华荠?，具有发汗解表、理气化湿之功效，主治夏月感冒、中暑呕恶，腹痛泄泻，疖肿等症。主要含有挥发油和黄酮等活性成分。目前有关石香薷的研究主要集中在其挥发油成分上，但临床用药以汤剂为主，所含挥发油成分较少，且研究表明其非挥发性部位具有显著的抗菌、抗病毒、抗氧化、抗炎等作用[1-3]。而目前有关其非挥发性部位有效成分提取工艺的研究未见报道。为充分开发和利用这一植物资源，本文采用响应面分析法，在前期单因素考察的基础上，考察了影响石香薷总黄酮回流提取效果的主要因素，确定了其最佳提取工艺条件。 　　1 仪器与材料 　　UV-2600A型紫外可见分光光度计：尤尼柯（上海）仪器有限公司；HANGPINGFA1104型电子天平：上海仪器厂。芦丁标准品购自中国药品生物制品检定所(批号:100226)