草鱼鱼鳞胶原蛋白提取分离工艺的分析-食品科学专业论文.docxVIP

i i Abstract Fish scales accounted for 2.5% of the total mass of the fish. Fish scales are rich in protein and various minerals. It mainly composed of protein and hydroxyapatite. Protein accounted for 50% to 70% of the total mass of fish scales .Protein mainly composed of collagen and keratin and a small amount of globulin. Although Chinas rich yield of freshwater fish, processing and utilization of fish scales is not enough and waste seriously. Collagen is a white, opaque, unbranched fiber protein, a molecular weight of 300,000 daltons.. Collagen is one of the major component of the human body to retain calcium, has an extremely important role in the bodys growth and development. Fish scales are rich in collagen, can be used as materials of collagen extraction. Use the grass carp scales as materials, explore the extraction technology of the collagen, and analysis the results. Fish scales mainly component of moisture, ash, impurity protein and a small amount of guanine and lecithin. The main chemical composition in fish scales were determined, results showed that moisture, ash, crude protein reached at 14.08%, 28.58%, 58.32%; the content of collagen reached at 51.24%. Decalcification technology: with the method of single factor design, results showed that best decalcification technology was EDTA concentration0.5mol/L, decalcification time24h, ratio of material to liquid 1:50, decalcification temperature 25℃, decalcification twice. The ultrasonic assisted decalcification results show that ultrasonic-assisted decalcification effect is not obvious. Remove impurity protein technology: with the method of single factor design, results showed that best remove impurity protein technology was ratio of material to liquid1:40, concentration of sodium carbonate0.3mol/L, the time of 6h, the temperature of 20℃, remove impurity three times. Ultrasonic-assisted is good for the result of remove impurity protein, at the ultrasonic frequency of 60Hz effact better. ASC extrac