促性腺激素释放激素激动剂预处理在兔卵巢组织玻璃化冷冻与自体移植中作用的实验分析-妇产科学专业论文.docxVIP

泡比例 GnRH 组较对照组高，差异有显著性（P0. 05）；移植物内卵泡比较，GnRH 组与对照组正常形态卵泡比例比较无显著性（P0. 05），正常形态卵泡比例均较移 植前显著降低（P0. 01），仅见少数原始卵泡和初级卵泡。 结论（1）腹股沟区大腿内侧肌肉层作为移植部位，简单易行，便于监测；（2）各 冻存组的卵巢自体移植后可以存活并具有内分泌功能；（3）GnRH- a 组卵巢功能恢 复所需要的时间更长，可能是由于 GnRH- a 抑制了卵泡启动；（4）GnRH- a 预处理 不能减少移植组织缺血- 再灌注损伤。 关键词 兔 卵巢冷冻 自体移植 促性腺激素释放激素激动剂 Par t Ⅰ Experimental Study on the Effect of the GnRH-a on Vitrification of Rabbit Ovarian Tissue Abst r act Objective:Observe differences of the injection of GnRH-a group and non-injection of GnRH-a group of the frozen-thawed ovarian tissue in rabbits at follicle morphology, quantity and apoptosis rate, to explore the possible effect of GnRHa pretreatment on rabbit ovarian tissue cryopreservation. Methods: (1) 18 female New Zealand white rabbits with normal estrous cycle were randomly divided into two groups：GnRH-a group(n=9) and non-injection of GnRH-a group (n=9).(2) GnRH-a group：injection of 2.5mg GnRH-a was treated every 15 days twice before ovarectomy. Then after ovarectomy ,one side of the ovary was vitrificated immediately after treatment(group A), the Other side of the ovary is not be frozen but drop into 4% paraformaldehyde fixed as control(group B); non-injection of GnRH-a group: injection of 0.3ml saline every 15 days,30 days later the ovarectomy was treated , one side of the ovary was vitrificated immediately after treatment(group C), the Other side of the ovary was not be frozen but drop into 4% paraformaldehyde fixed as control(groupD).(3) groupA and groupC have the same vitrification method using modified Noriko.K’s . (4) Histologically examined the morphological of follicles and qauntity of various stage follicles;Detected the apoptosis positive follicles in ovarian tissue by TUNEL． Result: (1) Histological examination: the proportion of morphologically normal follicles in groupA, groupB, groupC and groupD were 71.37%, 84.85%, 66.57% and 82.93%, the proportion of morphologically normal follicles cryopreserved group were lower than those of fresh group(