促红细胞生成素对兔视网膜神经节细胞的保护作用-眼科学专业论文.docxVIP

Abstract Objectiv To observe the neuroprotection of erythropoietin on rabbit retinal ganglion cells in chronic ocular hypertention model． Methods 35 rabbits without eye diseases were divided into 3 groups：groupI：normal group(5rabbits)，groupII：erythropoietin treated group(15 rabbits)，groupIII：balanced salt solution treated group(15 rabbits)．Rabbits in group II and III were induce III left eye by forcing pefusion of balanced salt solution into the anterior chamber as chronic ocular hypertenfion model．Right eyes were self-control eyes．Not standard eyeballs were discard andl0 eyeballs were selected and fixed in each group．(The retinal ganglion cells were detected by HE staining .The apoptosis cells were detected by TdT-dUDP terminal nick end-labeling (TUNEL)technique． Results The RGCs quantity of model eyes in group II and III were less than that of group I and self-control eyes，there were significant diferences(P0.05)．But the RGCs quantity of model eyes in groupII were more than that of groupIII，there were significant diferences(P0.05)．The TUNEL-positive cells of model eyes in group II and III were more than that of group I and self-control eyes，there were significant diferences(PO.05)．But the TUNEL-positive cells of model eyes in group II were less than that of groupIII，there were significant diferences(PO.5). Condusion erythropoietin could provide neuroprotection for RGCs in rabbit chronic glaucoma model． Key words：glaucoma；retinal ganglion cells(RGCs);EPO 主要符号表 英文缩写 EPO 英文全称 erythropoietin 中文译名 促红细胞生成素 EPOR erythropoietin receptor 促红细胞生成素受体 rHuEPO recombinant human erythropoietin 重组人促红细胞生成素 WHO World Health Organization 世界健康组织 RGC RGCL NMDA retinal ganglion cell retinal ganglion cell layer N-methyl-D-aspartate 视网膜神经节细胞 视网膜神经节细胞层 N-甲基-D-天冬氨酸 BDNF brain-derived neurotrophic factor 脑源性神经营养因子 NGF nerve growth factor 神经生长因子 CNTF ciliary neurotrophic factor 睫状神经营养因子 VEGF vascular endothelial growth factor 血管内皮生长因子 NO nitric oxide synthase 一氧化氮 石河子大学学位论文独创性声明及使用授权声明 学位论文独