促红细胞生成素治疗肾脏缺血再灌注损伤最佳剂量及时机的探讨-内科学专业论文.docxVIP

Optimal dosing and timing of erythropoietin for treatment of renal ischemia-reperfusion injury ABSTRACT Objective To evaluate different dosing and timing of recombinant human erythropoietin (rHuEPO) on renal ischemia reperfusion injury. Methods Sixty SD rats were randomly divided into six groups including sham operation (Sham) group, ischemia reperfusion injury (I/R) group, injection rHuEPO with 5000U/kg and procure specimens after 24h, injection rHuEPO with 2500U/kg and procure specimens after 24h, injection rHuEPO with 5000U/kg and procure specimens after 48h and injection rHuEPO with 2500U/kg then after 24h give an additional and procure specimens after 48h. Results Compared with Sham group, other rats have kidney damage, definition as significantly increased of BUN and Cr, apoptosis increased, p-JNK expression increased. With I/R group, other groups with the rHuEPO therapy have improved markedly in renal function. The group injection rHuEPO with 5000U/kg and procure specimens after 24h have the best results (P 0.05). Conclusion The timely and sufficient dose of rHuEPO on renal ischemia reperfusion injury has a better protective effect. Key Words: rHuEPO; reperfusion injury; p-JNK; dosing and timing ５ 英汉缩略词对照表 英文缩写 英文全称 中文全称 rHuEPO recombinant human erythropoietin 重组人红细胞生成素 ARIRI Acute renal ischemic reperfusion injury 急性肾脏缺血再灌注 损伤 ARF Acute Renal Failure 急性肾功能衰竭 p-JNK P-c-Jun N-terminal kinase 磷酸化应激活化蛋白 激酶 MAPK Mitogen-activated protein kinase 丝裂原活化蛋白激酶 Scr Serum creatinine 血清肌酐 BUN Blood urea nitrogen 血清尿素氮 HE hematoxylin eosin 苏木素-伊红 PBS Phosphate buffer saline 磷酸盐缓冲液 SDS Sodium dodecyl sulfate- Polyacrylamide gel electrophoresis 十二烷基硫酸钠-聚 丙稀酰胺凝胶电泳 TUNEL TdT-mediated dUTP nick end labeling TdT 介导的 dUTP 缺 口末端标记技术 AI apoptosis index 凋亡指数 SPSS Statistical Product and Service Solutions 社会科学统计软件包 OFR oxygen free radical 氧自由基 ６ 前言 临 床 上 急 性 肾 脏 缺 血 再 灌 注 损 伤 (Acute renal ischemic reperfusion injury，ARIRI)是急性肾衰（ARF）的常见原因，其病死率高达 50-70% [1]。 就目前而言，认为 ARIRI 的病理机制包括