发夹式siRNA慢病毒表达载体构建及对人SR-PSOX基因表达的影响-内科学(心血管病学)专业论文.docxVIP

Results The shRNA lentiviral expression vector targeting human SR-PSOX gene was constructed successfully. The expression levels of mRNA and protein of human SR-PSOX gene was significantly suppressed by the expression vector, the inhibition ratio could reach 80%. And it can induce the silence of human SR-PSOX gene efficiently in cultured cells. Conclusions The shRNA lentiviral expression vector targeting human SR-PSOX gene was constructed successfully. The expression of human SR-PSOX gene was efficiently suppressed by the lentiviral expression vector in cultured cells, thus lay the foundation for studying the function of the gene using RNAi technology. Postgraduate: Peng Hui-bing (cardiovascular medicine) Directed by: Prof. Quan Zhi-hua Key words shRNA ; vector construction; human SR-PSOX gene ; RNA interference 目 录 英文缩略词Ⅲ 中文摘要1 英文摘要2 前言4 材料与方法5 结果21 讨论26 结论28 参考文献29 文献综述31 攻读学位期间所发表的文章41 致谢42 主要英文缩略语 缩略语 英文全称 中文译名 RNA Ribonucleic acid 核糖核酸 RNAi RNA interference 核糖核酸干扰 siRNA small interference RNA 小干扰 RNA shRNA small hairpin RNA 发夹式 siRNA RISC RNA-induced silencing complex 核糖核酸诱导沉默复合物 SEC siRNA expression cassette 小干扰核糖核酸表达盒 dsRNA Double-standed RNA 双链核糖核酸 mRNA Messager RNA 信使核糖核酸 DNA Deoxyribonucleic acid 脱氧核糖核酸 dNTP Deoxyribonucleoside triphosphate 脱氧核糖核苷三磷酸 Bp Base pair 碱基对 cDNA complementary DNA 互补脱氧核糖核酸 EB Ethidium Bromide 溴化乙锭 PBS Phosphate buffer solution 磷酸盐缓冲溶液 PCR Polymerase chain reaction 多聚酶链反应 RT-PCR Reverse transcription PCR 逆转录多聚酶链反应 IFL indirect immunofluorescence 间接免疫荧光法 Taq Thermus aquaticus DNA Polymerase 栖热水生菌 DNA 聚合酶 Tris Tris(hydroxymethyl)aminomethane 三羟甲基氨基甲烷 Tris-HCl Tris-hydrochloric acid buffer Tris-盐酸缓冲液 LB Luria-bertani(medium) LB 培养基 DMSO dimethyl sulphoxide 二甲基亚砜 AS atherosclerosis 动脉粥样硬化 DEPC Diethylpyrocarbonate 焦碳酸二乙酯 HRP horseradish peroxidase 辣根过氧化物酶 ox-LDL oxygen-low-density lipoprotein 氧化型低密度脂蛋白 PVDF polyvinylidene difluoride 聚丙二氟乙烯 CXCL16 CXC chemokine ligand 16 CXC 亚族第 16 号趋化因子 SDS 一 PAGE： sodium dodecyl su