促甲状腺激素(TSH)对裸鼠甲状腺乳头状癌组织的影响及意义-外科学专业论文.docxVIP

目 录 中文摘要 ····································································································· 1 英文摘要 ····································································································· 4 研究论文 促甲状腺激素对裸鼠甲状腺乳头状癌组织的影响及意义 前言 ····································································································· 8 材料与方法 ······················································································· 10 结果 ··································································································· 17 附图 ··································································································· 19 附表 ··································································································· 25 讨论 ··································································································· 26 结论 ··································································································· 29 参考文献 ··························································································· 30 综述 甲状腺乳头状癌的术后辅助治疗 ················································· 33 致谢 ··········································································································· 45 个人简历 ··································································································· 46 促甲状腺激素(TSH)对裸鼠甲状腺乳头状癌组织的影响及意义 摘 要 目的：甲状腺乳头状癌术后患者长期服用超生理需要量的甲状腺素会 产生主要针对心血管和骨骼的不良影响。长期促甲状腺激素抑制会使患者 心率加快，频发的房性期前收缩。而绝经后妇女促甲状腺激素≤0.03mU/L， 骨质疏松导致骨折的风险增加 2 倍[1]。甲状腺乳头状癌中约有 10%的患者 出现复发，其中只有一小部分会因复发而死亡，甲状腺乳头状癌患者的促 甲状腺激素水平到底应该抑制到多少能够使患者受益还尚未有明确的研 究可以说明。本研究旨在探讨不同浓度的促甲状腺激素对甲状腺乳头状癌 生长的作用，为促甲状腺激素抑制的临床用药提供理论依据。 方法：35 只体重在 7~9 克直间的雄性 BALB/c-nu/nu 裸小鼠按照完全 随机化原则分为 7 组。取人乳头状甲状腺癌细胞系 TPC-1，以 1×106 细胞 数制成 100ul 细胞悬液接种于 35 只裸小鼠皮下，并分别给予不同浓度的 TSH：对照组(0mU/L)、2mU/L、4mU/L、8mU/L、12mU/L、16mU/L、20mU/L。 观察并测量各组瘤体大小，并用放射免疫分析法测定瘤体组织匀浆中甲状 腺球蛋白(TG)的含量。 结果： 1 甲状腺乳头状癌细胞的培养 将培养瓶放于 37℃，5%浓度的 CO2 的培养箱内培养。4-5d 后，大量 细胞贴壁。进入甲状腺乳头状癌细胞的潜伏期，期间细胞的伪足进一步伸 长，细胞