创复康喷剂对大鼠创伤感染模型创面抗感染及NO含量影响的研究-中医骨伤科学专业论文.docxVIP

摘 要 目的：通过观察创复康喷剂对大鼠感染模型创面中抗感染、体外抑菌及 NO 含量的 影响，旨在从微生物学及分子生物学角度探讨创复康喷剂对感染创面修复作用的 机制。 方法：（1）30 只健康 SD 大鼠，雌雄各半，随机分为 A 生理盐水空白组、B 创复康 喷剂实验组、C 康复新液药物对照组，每组 10 只，称重标记。按照付小斌等报道的 背部全层皮肤切除感染模型制备方法并加以改进，制作大鼠感染模型。 （2）造模成功后开始分组外喷创复康喷剂、康复新液、生理盐水，5ml/次，每日2 次，连续用9日。在治疗第1d、5d、9d，分别于创面中心及边缘各切取一痂下组织 或肉芽组织，制成组织匀浆，进行菌落计数。用药第3d、7d，切取未愈合创面边 缘0.3cm的新生肉芽组织和皮肤，每只大鼠取约0.5g，超声波粉碎后，制成组织 匀浆，将制备好的匀浆离心留上清液，采用比色法先测定组织蛋白含量再测定匀 浆液NO 含量, 操作步骤严格按考马斯亮兰和NO 试剂盒说明进行。实验结果根据标 准品的浓度及对应的OD值，计算出标准曲线的回归方程，再根据样本的OD值，在回 归方程上计算出对应的样品浓度，也可以使用应用软件来计算。最终浓度为实际测 定浓度乘以稀释倍数。 结果：（1）创复康喷剂组和康复新液组均能能降低感染创面中细菌含量，无显 著性差异，在治疗后第 5d、9d，创复康喷剂组和生理盐水组的细菌含量有显著性 差异。表明运用创复康喷剂可以降低感染创面中细菌含量，有抗感染的作用。 （2）用药 3d、7d 后，创复康喷剂组和康复新剂组的 NO 含量无显著性差异， 创复康喷剂组与生理盐水组的 NO 含量有显著性差异。 结论：创复康喷剂能够降低感染创面中细菌含量，能促进感染创面的愈合,提高 NO 的含量。 关键词：创复康喷剂；全层皮肤切除模型；体外抑菌；NO；细菌含量。 The content of the bacterial and (NO) affected by wound healing spray on the wound on wound infection in rats Abstract Purpose ： Record of rehabilitation by observing the spray on the wound infection model in rat anti-infective, NO content, designed from the perspective of microbiology and molecular biology of invasive wound infection rehabilitation spray repair mechanism. Method: 1) 30 healthy SD rats were randomly divided into saline control group A, B Chong Rehabilitation spray the experimental group, C rehabilitation new fluid control group, n = 10, weighing tag. Such reports in accordance with Fu Xiaobin back full thickness skin excision infection model and improved preparation, rat infection model. (2) After the modeling group and Spraying started spray invasive rehabilitation, rehabilitation of new fluid, normal saline, 5ml / times, 2 times a day for successive 9. In the treatment section 1d, 5d, 9d, respectively, the edge of the wound center and take a cut or granulation tissue under eschar, made of tissue, for colony counting. Medication section3 d and 7d, cut off the edge of the wound did not heal 0.3cm of new granulation tissue and skin, each rat was taken about 0.5g, ultrasonic crushed, mad