反义uPAR质粒对鼠RA实验性治疗的初步研究-外科学专业论文.docxVIP

Object:  Abstract According to the changes that secondary paw swelling, polyarthritis, and pathomorphology change in RA, this study tends to clarify the therapeutic effect of antisense uPAR expressing plasmid in AA rats. According to the investigation the expression of the uPAR and VEGF in synoviocytes, analysis the therapeutic effect of the antisense uPAR expressing plasmid through affect the uPAR and VEGF in synoviocytes. Methods: Extraction plasmid: Using the traditional alkaline method to isolate pcDNA3.1(-) plasmid and antisense pcDNA3.1(-) plasmid DNA from the bacterial solutions. Duplication and treatment of AA rats model: After one weeks adaptability breeding with 50 male SD rats, the rats were divided into normal control group (n=8) and Adjuvant arthritis group (n=42) by completely random. The normal control group rats weren’t received any treatment but the AA rats was made by inoculating complete freund’s adjuvants (CFA) in the left foot. After the AA model was made successfully, the AA rats were randomly divided into 4 groups: 8 as model control group, 8 as physiological saline treatment group by injecting physiological saline, 8 as empty plasmid treatment group by injecting pcDNA3.1(-) plasmid, 8 as recombinant plasmid treatment group by injecting antisense pcDNA3.1(-) plasmid. Observation of result: All the rats were selected to observe the general condition, arthritis index (AI) , the histopathological changes of synovium with optical microscope and the expression of uPAR and VEGF detected by immunohistochemical method. Results: The result of plasmids:After extraction of plasmids, agarose gel electrophoresis showed that the recycling plasmids and the DNA marker were in the rather right position; The plasmids was considered to be in good purity by ultraviolet spectrophotometer. The extraction of plasmids was succeed. General observation: With the time lasting, the arthritis index of model control group, physiological saline treatment group and empty p