促红细胞生成素在脉络膜新生血管中的作用-眼科学专业论文.docxVIP

Abstract Purpose: To investigate the role of erythropoietin (Epo) in the pathogenesis of experimental choroidal neovascularization (CNV) and effect of anti-Epo therapy in CNV. Methods: The 2-3 months old health C57BL/6 mice were divided into 3 groups: laser treatment group, laser treatment + Epo siRNA injection group, laser treatment + control siRNA injection group. In laser induced CNV mice model, the expression of Epo in CNV tissue was evaluated by western blotting and immunohistochemistry on the 0d, 3d, 7d, 10d, 14d, 17d after laser treatment. Epo siRNA was injected intravitreally into the laser induced CNV mice model, the expression of Epo and the area of neovascularization were accessed by western blotting and immunohistochemistry respectively on the 7d after laser treatment. Results: The expression of Epo was upregulated in laser induced choroidal neovascularization tissue. The highest expression of Epo is about 3.8 folds increase of baseline at 10 days after laser treatment. It was showed by immunohistochemistry that Epo expressed on CNV tissue around endothelial cells. SiRNA targeting Epo reduced about 40% expression of Epo and 40% of area of CNV. Conclusion: The expression of erythropoietin is increased in laser induced choroidal neovascularization tissue. Epo siRNA decreased expression of Epo and reduced the area of choroidal neovascularization. These results suggest that erythropoietin may play an important role in the pathogenesis of choroidal neovascularization. Erythropoietin may be a novel therapeutic target for choroidal neovascularization. 缩略词表 缩略语 Epo 英文全称 Erythropoietin 中文全称 促红细胞生成素 AMD Age-related marcular degeneration 年龄相关性黄斑变性 CNV Choroidal neovascularization 脉络膜新生血管 C57BL/6 DAPI EPOR C57BL/6 4,6-diamidino-2-phenylindole Erythropoietin receptor C57BL/6 小鼠 4,6-二脒基-2-苯基吲哚 促红细胞生成素受体 GFAP Glial fibrillary acidic protein 胶质纤维酸性蛋白 HIF Hypoxia inducible factor 缺氧诱导因子 IGF Insulin-like growth factor 胰岛素样生长因子 RPE Retinal pigmental ep