二甲双胍和游泳运动对肥胖大鼠血清Visfatin及肾周脂肪等Visfatin蛋白表达水平的影响-内分泌与代谢病专业论文.docxVIP

继续予以高脂饲料喂养。MET 组予二甲双胍灌胃 200mg/kg.d，SWI 组进行游泳运 动，MAS 组予二甲双胍灌胃 200mg/kg.d 联合游泳运动，SWI 组、OB 组及 ND 组予 以等剂量蒸馏水灌胃，共 6 周，测定干预前后各组体重(BW)、空腹血糖(FBG)、甘 油三酯(TG)、总胆固醇(TCH)、胰岛素(FINS)及血清 Visfatin 水平。利用 Western Blot 法检测大鼠皮下脂肪、肾周脂肪和肌肉组织各组别 Visfatin 蛋白表达水平。 结果 ① 干预前，肥胖模型各组 BW、FBG、TG、TCH、FINS 及 Visfatin 水平高于 ND 组(P 0.01 或 P0.05)。② 干预后，MET 组、SWI 组、MAS 组 BW、TG、TCH、 FBG、FINS 及 Visfatin 较 OB 组降低 (P 0.01 或 P0.05)。③OB 组肾周脂肪、皮 下脂肪及肌肉组织的 Visfatin 蛋白表达量较 ND 组对应组织 Visfatin 蛋白表达量增 加(P 0.01)，以肾周脂肪显著(P0.05)，OB 组皮下脂肪与肌肉组织 Visfatin 蛋白 表达量无差异。MET 组、SWI 组、MAS 组肾周脂肪和皮下脂肪 Visfatin 蛋白表达 水平较 OB 组对应组织 Visfatin 蛋白表达量降低，以 MAS 组显著(P0.05)，MET 组与 SWI 组差异无统计学意义。MET 组、SWI 组、MAS 组与 OB 组 Visfatin 蛋 白表达在肌肉组织无差异，干预组间差异亦无统计学意义。④BW、TG、TCH、 FBG、FINS 水平与 Visfatin 水平呈正相关(r=0.86, P0.01; r=0.83, P0.01; r=0.88, P0.05; r=0.91, P0.05; r=0.85, P0.05 )；血清 Visfatin 水平与肾周 Visfatin 蛋白表达 量呈正相关(r=0.95, P0.05 ) 结论 Visfatin 主要表达于内脏脂肪，参与肥胖大鼠的糖脂代谢，二甲双胍和游泳运 动可下调肥胖大鼠血清、皮下脂肪和肾周脂肪 Visfatin 蛋白的表达，对肌肉组织 Visfatin 蛋白表达无影响。 关键词：二甲双胍 游泳运动 内脂素 肥胖 代谢综合征 Impact of Treatment with Metformin and Swimming Exercise on Serum Visfatin and Visfatin Protein Expression Levels in Perirenal adipose tissue of High-fat-diet-fed Obesity Rats Abstract Background Visfatin known previously as pre-B cell colony-enhancing factor is the most recently identified peptide expressed in human visceral and subcutaneous adiopose tissue with a molecular weight of 52KD. Its amino acid sequence is highly conserved in each species and Visfatin is also widely expressed in muscle, liver, marrow and macrophage. Visfatin mimics the action of insulin through a distinct binding site on the insulin receptor, affects lipid profiles and promote the synthesis, differentiation and accumulation of adiopose tissue. Visfatin also plays roles in inflammation and the pathophysiology of atherosclerosis, regulates the maturity of vascular smooth muscle, and is a new bond linking glucolipid metabolism disorders and cardioascular diseases. Insulin-sensitizing agent Metformin is a widely used drug for