传统炮制工艺对土家药“隔山消”抗肿瘤作用和毒性的影响-生化与分子生物学专业论文.docxVIP

摘 要 传统土家药隔山消有抗肿瘤的功效，但长期以来关于其有毒与否、毒性大小 的问题存在争议。为减毒增效，在使用时土家医往往要对隔山消进行炮制，不同 地区的土家医对隔山消的炮制方法也不同，经调研发现对隔山消的炮制目前主要 存在着两种方法：米炒法和黑豆黄酒制法。本文通过建造荷 S180 肉瘤小鼠的整体 动物模型评价了传统炮制工艺对隔山消及其总苷部位的抗肿瘤作用和毒性的影 响。希望能为隔山消的临床应用提供相关实验依据。主要结果如下： 1.对小鼠 S180 肉瘤的抑制率 隔山消水提物的最大抑制率均出现在高剂量组，分别为：生品 54.64%、炮制 品 1 54.22%、炮制品 2 46.93%；总苷的最大抑制率分别为：生品高剂量组 68.99%、 炮制品 1 中剂量组 79.04%、炮制品 2 中剂量组 68.55%。生品和炮制品总苷对小 鼠 S180 肉瘤的抑制率明显高于水提物。 2.免疫器官指数 隔山消生品和炮制品的水提物对小鼠的脾脏指数和胸腺指数无明显的毒副作 用，与生理盐水组小鼠的脾脏指数和胸腺指数处于同一水平；总苷对免疫器官指 数影响显著。 3.血常规和肝肾功能 隔山消炮制前后水提物和总苷对小鼠血常规各项指标无不良影响。水提物各 给药组白细胞水平均高于生理盐水组。较之生理盐水组，水提物各给药组小鼠的 肝肾功能无显著性差异，总苷各组小鼠肝肾功能多数异常且出现显著性差异。 4.急性毒性 实验中使用的水提物和总苷对小鼠没有急性毒性。 关键词：炮制；隔山消；总苷；小鼠 S180 肉瘤；抑瘤率；毒性 Abstract The tradiational Tujia herbal medicine Cynanchum auriculatum Royleex Wight (RCA) has the antitumor activity,but there is a disput about wether it is toxic or not ever since a long time ago. Animal experiment were carried out with the water extractive,total glycosides from fresh and different processing methods of RCA.With the hope that could provide a experimental foundation of scientific and standerd using of this herbal.The main results : The Anti—tumor activity to Sl80 tumor The max Anti—tumor activity of water extractive are all in each high dose group,were: crude group 54.64%,processing 1 group 54.22%, processing 2 group 46.93%；The max Anti—tumor activity of total glycosides were:crude high group 68.99%, processing 1 middle group 79.04%, processing 2 middle group 68.55%. The Anti — tumor activity of total glycosides are all significant strongger than water extractive. Immune Organ Index The water extractive and total glycosides of crude and different processing RCA has no adverse effects on Spleen index and Thymus index of mice;has no adverse effects on Spleen index and Thymus index like cisplatin; The Spleen index and Thymus index are at a same level as NS group. Blood routine,Liver and Kidney functinos The water extractive and total glycosides of crude and different processin