恶性胸腔积液中NK和γδT细胞的表型与功能研究-免疫学专业论文.docxVIP

二、γδT 细胞的表型与功能改变 与配对外周血相比，MPE 中γδT 细胞的表型与功能有如下改变：①γδT 细胞比例 下降，且 CD4+亚群比例上升。②γδT 细胞产生 IFN-γ减少，产生 IL-4 增多。③γδT 细 胞活化受体 CD226 表达降低。④γδT 细胞胞毒活性减弱。 三、胸水上清刺激导致 NK 与γδT 细胞胞毒活性减弱 恶性胸水上清刺激健康人 PBMC 24h，可导致 CD56dim NK 和γδT 细胞胞毒活性受 损。提示胸水中某些成分可以影响 NK 与γδT 细胞的活性。 结论： 与配对外周血相比，MPE 中 NK 与γδT 细胞在比例，亚群，表面活化受体，胞内 细胞因子分泌的能力均发生变化，胞毒活性均下降。上述改变可能是胸水上清中某些 成分所致，其机制有待深入研究。 关键词：NK 细胞；γδT 细胞；MPE；CD226；IFN-γ；IL-4；胞毒活性 Phenotypic and functional study of NK and γδT cells in malignant pleural effusion Master Candidate：Qi Jing Tutor：Associate Prof. Zheng Fang Prof. Gong Feili Department of Immunology, Tongji Medical College of Huazhong University of Science and Technology Abstract Malignant pleural effusion (MPE) is caused by primary or metastatic tumors at the pleura. MPE is a common complication observed at the advanced stage of various malignant diseases. An accumulation of lymphocytes, especially CD4+ T lymphocytes, frequently occurs in MPE. MPE offers a unique platform to study specific interactions between the immune cells and tumors. Most studies have focused on CD4-positive T lymphocytes recently, but pay little attention to the innate immune cells in MPE. NK and γδT cells are important cells of innate immune system. They play crucial roles in recognition and elimination of tumors. Earlier study demonstrated that NK cells in MPE displayed decreased cytotoxicity against tumor cells. It suggested that NK cells might involve in the genesis and development of MPE. In addition, there are no reports about the phenotype and function of γδT cells in MPE at present. Objective: To investigate the phenotype, function and the related mechanism of NK and γδT cells in MPE from patients with lung cancer. Methods: Mononuclear cells in MPE and paired peripheral blood were separated with Ficoll-hypaque density gradient centrifugation. The proportion, surface activating receptors, intracellular cytokines and cytotoxic activity of NK and γδT cells were detected by flow cytometry. Furthermore, PBMC from