创复康喷剂对大鼠创伤感染模型创面中细菌及TGF α含量影响的研究-中医骨伤科学专业论文.docxVIP

The research of The content of the bacterial and (TGF-α)affected by wound healing spray on the wound infection in rats Abstract Objective ： To confer the repairing mechanisms of wound healing spray on invasive infection from the perspective of microbiology and molecular biology through observing the bacterial and transforming growth factor-α (TGF-α) content affected by wound healing spray on rat Methods: (1) 30 healthy SD rats were randomly divided into group A normal saline as blank, group B wound healing spray as the experimental, group C would rehabilitation new fluid as control, n = 10, with weighing tag . Make rat infection model according to reports by Fu Xiaobin that back full thickness skin excision infection model and make some improvement. (2) All the rats devided into normal saline group, wound healing spray group and would rehabilitation new fluid group after getting the model. 5ml / times, 2 times a day for successive 8. On the treatment of the 0d, 4d, 7d, respectively, a callus or granulation tissue was excised form the center or the edge of the wound ,made into homogenate for colony counting. On the 7d, new granulation tissue and skin was excised form 0.2cm edge of the wound which did not heal. 0.5 g was taken from each rat, after ultrasonic fragmentation, made into tissue homogenate ,keep the supernatant after centrifugaling tissue homogenate ,then detected the concentration of TGF-α by radioimmunoassay. The results was analyzed by SPSS 16.0 statistical software, all the data information with completely randomized design analysis of variance results. Results：(1) Both wound healing spray group and would rehabilitation new fluid group can reduce bacteria in wound infection with no significant differences in treatment. after 4d, 7d. wound healing spray group and saline group has significant differences in content of bacteria, Showing that the use of wound healing spray can reduce bacteria in wound infection, having effect of anti-infection. (2) There w