雷利度胺抗胃癌细胞的作用及机制的研究.docVIP

雷利度胺抗胃癌细胞的作用及机制的研究 　　[摘要] 目的 探讨雷利度胺对胃癌MFC细胞体外增殖与凋亡的影响及其作用机制。 方法 以MTT法检测雷利度胺对MFC细胞增殖的影响，荧光显微镜观察雷利度胺作用后MFC细胞形态的变化，流式细胞仪检测雷利度胺作用后MFC凋亡率，Western blot分析凋亡相关蛋白Bcl-2、Caspase-3表达。 结果 雷利度胺能显著抑制MFC细胞的增殖，呈现出剂量效应及时间效应趋势。雷利度胺作用MFC细胞后呈现典型细胞凋亡的形态学改变、凋亡增加。与对照组比较，60 mg/L雷利度胺诱导72 h组及120 mg/雷利度胺诱导72 h组的细胞可以明显抑制MFC细胞的生长（P 0.01）。与对照组比较，雷利度胺作用后Bcl-2表达明显下降（P 0.01），Cleaved Caspase-3表达有所增加（P 0.05）。 结论 雷利度胺可抑制MFC细胞增殖、促进细胞凋亡，其机制可能与激活Caspase-3、下调Bcl-2表达有关。 　　[关键词] 雷利度胺；MFC细胞；增殖；凋亡 　　[中图分类号] R735.2 [文献标识码] A [文章编号] 1673-7210（2016）12（c）-0018-04 　　[Abstract] Objective To explore the effects of Lenalidomide on gastric carcinoma MFC cells proliferation and apoptosis in vitro and its mechanism. Methods The inhibitory effect of Lenalidomide on MFC cells was estimated by MTT assay， and the morphological changes of the cells were observed under fluorescence microscope. Cell apoptosis were analyzed using flow cytometry. The protein expressions of apoptosis-associated genes as Bcl-2 and Caspase-3 were viewed by Western blot. Results Proliferation of MFC cells was inhibited effectively after treated with Lenalidomide. Lenalidomide inhibited the proliferation of MFC cells in a time- and dose-dependent manner. Lenalidomide treatment resulted in changes of apoptotic morphology of the cells as observed under fluorescence microscope and increased cell apoptosis. Compared with control group， 60 mg/L Lenalidomide group and 120 mg/L Lenalidomide group can significantly inhibit the growth of MFC cells after treatment of 72 hours （P 0.01）. Compared with control group， the expression of Bcl-2 was significantly decreased after Lenalidomide （P 0.01）， the expression of Cleaved Caspase-3 was increased after Lenalidomide （P 0.05）. Conclusion Lenalidomide can inhibit MFC cell proliferation， induce cell apoptosis， and the mechanism may be correlated to the up-regulation of Caspase-3 and down-regulation of Bcl-2. 　　[Key words] Lenalidomide； MFC cell； Proliferation； Apoptosis 　　雷利度胺是沙利度胺的?似物，最早用于多发性骨髓瘤[1]的治疗，目前在