Rh／RK信号通路在腹膜透析大鼠腹膜纤维化中的作用及法舒地尔的干预效应.docVIP

Rho/ ROCK信号通路在腹膜透析大鼠腹膜纤维化中的作用及法舒地尔的干预效应 孟绪初1甘平2(通讯 ) (1贵州医科大学研究生学院贵州贵阳550004) (2贵州医科大学附属乌当医院肾内科贵州贵阳550018) 】目的：探讨Rho/ROCK信号通路在腹膜透析大鼠腹膜纤维化屮的 可能机制。方法:将30只雄性SD大鼠随机分为四组：对照组，每日腹腔注射生 理盐水25ml;模型组，每口腹腔注射4.25%的腹透液25ml; Fasudil组，每口腹 腔注射含法舒地尔(Fasudil)浓度为10mg/Kg?d的4.25%腹透液25ml。实验第28 天，取壁层腹膜组织行HE染色，并测定壁层腹膜ROCK-1的表达情况及SOD、 MDA含量。结果：与模型组比较，Fasudil组不同程度腹膜厚度减少、胶原纤维 沉积减轻，腹膜组织ROCK-1表达减少(Plt;0.05)，MDA含量降低(Plt;0.05), SOD 活性增加(Plt;0.05)。结论：腹膜透析液激活丫腹膜透析人鼠腹膜Rho/ROCK信 号通路，促进腹膜组织氧化应激损伤，导致丫腹膜纤维化的发生，ROCK抑制剂 法舒地尔能通过抑制此通路减少腹膜的氧化应激反应，进而延缓腹膜纤维化进展。 关键词】腹膜透析；腹膜纤维化；氧化应激；Rho/ROCK信号通路 屮图分类号】R459.5 】A 】2095-1752 (2016) 08-0059-03 Rho/ROCK signaling pathway in pd rat peritoneal fibrosis, the effect of the intervention effect of shu, and the method Meng Xuchu 1 GanPing 2.1Guizhou GuiZhou GuiYang medical University graduate school of 550004,China.2Wu when Hospital affiliated to medical University renal medicine in guizhou GuiZhou GuiYang 550018,China Abstract 】Objective To study the Rho/ROCK signaling pathway in the possible mechanism of pd rat peritoneal fibrosis. Methods 30 male SD rats were randomly divided into four groups: control group, the daily intraperitoneal injection of saline 25 ml; Model group, the daily intraperitoneal injection of 4.25% of the liquid to drain 25 ml; By intraperitoneal injection of Fasudil group, daily with shu to farmar (Fasudil) concentration of 10 mg/Kg, 4.25% of the d to drain fluid 25 ml. Experiment 28 days, parietal layer and organizational line HE staining, and the determination of parietal layer and the expression of ROCK -1 and SOD, MDA content. Results Compared with model group, different Fasudil group peritoneal thickness reduce, collagen fibers deposition reduction, reduced peritoneal organization ROCK -1 expression (P lt; 0.05), lower MDA content increased (P lt; 0.05), SOD activity (P lt; 0.05). Conclusion Peritoneal dialysate activates the pd rat peritoneal Rho/ROCK signaling pathway, promote the peritoneal tissues oxidative stress damage, caused the