desialylation is associated with apoptosis and phagocytosis of platelets in patients with prolonged isolated thrombocytopenia after allo-hsct：脱唾液酸与延长患者的血小板分离血小板减少和吞噬凋亡的异基因造血干细胞移植后.pdf

Zhang et al. Journal of Hematology Oncology (2015) 8:116 DOI 10.1186/s13045-015-0216-3 JOURNAL OF HEMATOLOGY ONCOLOGY RESEARCH Open Access Desialylation is associated with apoptosis and phagocytosis of platelets in patients with prolonged isolated thrombocytopenia after allo-HSCT *† † Xiao-Hui Zhang , Qian-Ming Wang , Jia-Min Zhang, Fei-Er Feng, Feng-Rong Wang, Huan Chen, Yuan-Yuan Zhang, * Yu-Hong Chen, Wei Han, Lan-Ping Xu, Kai-Yan Liu and Xiao-Jun Huang Abstract Background: Prolonged isolated thrombocytopenia (PT) is a frequent complication in patients who undergo allogeneic hematopoietic stem cell transplantation (allo-HSCT), and it is associated with an adverse prognosis. In this study, we hypothesized that desialylation on platelet surfaces was associated with PT after allo-HSCT. The mechanisms participating in this process may include NEU1 translocation, platelet apoptosis, and phagocytosis by macrophages. Methods: PT was defined as a peripheral platelet count less than 100 × 109/L without sustained anemia or leukopenia for more than 3 months after allo-HSCT. 34 patients were identified consecutively from a cohort of 255 patients who underwent allo-HSCT for hematologic malignancies between May and October 2014 at Peking University Institute of Hematology. Desialylation, enzyme expression, and phagocytosis were detected using flow cytometry, immunofluorescence, RT-PCR, Western blot, and so on. Results: Platelets from the PT patients had