成人髓核细胞特征性表达基因的筛选与验证-外科学(骨外)专业论文.docx

SDS Sodium dodecyl sulfate 十二烷基硫酸钠 SNAP25 synaptosomal-associated protein 25 突触体相关蛋白 25 SPSS Statistical Product and Service Solutions 统计产品与服务解决方案 TAE Tris acetic acid EDTA buffer 三羟甲基氨基甲烷醋酸 EDTA 缓冲液 TEMED N,N,N`,N`-tetramethylethylenediamine 四甲基二乙胺 Tris Trihydroxymethlaminomethan 三羟甲基氨基甲烷 μl Microlitre 微升 μg Microgramme 微克 2 The Screening and Identification of Marker Gene of Human Nucleus Pulposus Cell Abstract Background and Objective Low back pain has been a major disturbance in people’s life. From some investigations, the main cause for low back pain is due to lumbar disc degenerative diseases. It is now generally accepted that increased stress on lumbar disc, decreased nutrition supply and genetic factor can lead to the degenerative change in intervertebral disc. Lumbar intervertebral disc is divided into 3 parts, nucleus pulposus(NP) located in the center, annulus fibrosus(AF) around nucleus pulposus and the cartilage endplate covered AF and NP, also sticked to the upper and lower bone endplate of vertebral body. The main function of lumbar disc is to resist the stress by hydrostatic pressure from the combination of typeⅡ collagen and aggrecan to water. Lumbar disc can also provide the movement of lumbar spine. The annulus fibrosus in the outer region can keep the inner nucleus pulposus in its location. The intervertebral disc is lack of blood supply, in this case, it can not acquire nutrition supply from blood vessels. As a result, the infiltration via upper and lower cartilage end plate can meet its nutritional demand. Once the degenerative changes take place, the cartilage endplate will be calcified and the infiltration of nutrition is decreased. Then, the nucleus pulposus cells can not absorb enough nutrition. In this way, the degenerative changes will take plac. Also, the over-loaded stress on the lumbar spine will lead to the changes of the compositions of the matrix. Consequently, the disc ability of resistance to stress is decreased. The l